Chemistry of carbon compounds – Miscellaneous organic carbon compounds – C-metal
Patent
1981-09-16
1983-01-18
Phillips, Delbert R.
Chemistry of carbon compounds
Miscellaneous organic carbon compounds
C-metal
C07C10352
Patent
active
043691382
ABSTRACT:
A method for the radioimmunoassay of parathyroid hormone in mammalian serum. The method is an improvement on previous double antibody radioimmunoassays in that spurious assay results caused by the nonspecific interaction of serum proteins with the labeled peptide is eliminated by the labeling of a specific portion of either human or bovine parathyroid molecule. The 65-84 portion of human or bovine parathyroid hormone, as radioactively labeled, is incorporated in the assay as the labeled peptide. A chicken antibody with a high affinity for the 65-84 portion of human parathyroid hormone is incorporated in the assay as the first antibody. The invention further pertains to compounds useful in practicing the method, namely X.sup.64 -hPTH.sup.65-84 and Y-X.sup.64 -hPTH.sup.65-84 wherein X is either histidyl, tyramyl, histamyl, or tyrosyl, and wherein Y is either .sup.125 I or .sup.131 I.
REFERENCES:
patent: 4146602 (1979-03-01), Gutcho et al.
Arnold W. Lindall, "Radioimmunoassay of Human Parathyroid Hormone", 10/78.
Jacqueline G. Parthemore, et al., "Asssessment of Acute and Chronic Charges in Parathyroid Hormone Secretions by a Radioimmunoassay with Predominant Specificity for the Carboxy-Terminal Region of the Molecule", 1978.
F. P. Di Bella et al., "Parathyrin Radioimmunoassay: Diagnostic Utility of Antisera Produced Against Carboxyl-Terminal Fragments of the Hormone from the Human", 1978.
European PTH Study Group, "Interlaboratory Comparison of Radioimmunological Parathyroid Hormone Determination", 1978.
Hunter Health III & F. P. Di Bella, "Reduced-Volume Radioimmunoassays for Parathyrin and Calcitonin in Serum, for Use in Pediatric and Small-Animal Studies", 1978.
F. P. Di Bella et al., "Carboxyl-Terminal Fragments of Human Parathyroid Hormone in Parathyroid Tumors: Unique New Source of Immunogens for the Production of Antisera Potentially Useful in the Radioimmunoassay of Parathyroid Hormone in Human Serum", 1978.
Claude Arnauld et al., "Radioimmunoassay of Human Parathyroid Hormone in Serum", 1971.
John M. Stewart & Janis D. Young, "Solid Phase Peptide Synthesis", pp. 1-26.
Bernard M. Jaffe & Harold R. Behrman, "Chapt. 19: Parathyroid Hormone," Methods of Hormone Radioimmunoassay.
Immuno Nuclear Corporation
Phillips Delbert R.
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