Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2006-02-21
2006-02-21
Fredman, Jeffrey (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091200, C435S091100, C536S023100, C536S024300, C536S024310, C536S024330
Reexamination Certificate
active
07001722
ABSTRACT:
A method of analyzing a polynucleotide of interest, comprising providing one or more sets of consecutive oligonucleotide primers differing within each set by one base at the growing end therof; annealing a single strand of the polynucleotide or a fragment of the polynucleotide to the oligonucleotide primers under hybridization conditions; subjecting the primers to single base extension reactions with a polymerase and terminating nucleotides, the terminating nucleotides being mutually distinguishable; and observing the location and identity of each terminating nucleotide to thereby analyze the sequence or a part of the nucleotide sequence of the polynucleotide of interest, is disclosed. An apparatus comprising a solid support to which is attached at defined locations thereon one or more sets of consecutive oligonucleotide primers differing within each set by one base at the growing end thereof is also described.
REFERENCES:
patent: 4689405 (1987-08-01), Frank et al.
patent: 4874492 (1989-10-01), Mackay
patent: 5202231 (1993-04-01), Drmanac et al.
patent: 5302509 (1994-04-01), Cheeseman
patent: 5503980 (1996-04-01), Cantor
patent: 5605794 (1997-02-01), Rust et al.
patent: 5631134 (1997-05-01), Cantor
patent: 5888819 (1999-03-01), Goelet et al.
patent: 6004744 (1999-12-01), Goelet et al.
patent: 6013431 (2000-01-01), Soderlund et al.
patent: 0 412 883 (1991-02-01), None
patent: WO 89/10977 (1989-11-01), None
patent: WO 90/04652 (1990-05-01), None
patent: WO 90/09455 (1990-08-01), None
patent: WO 90/13666 (1990-11-01), None
patent: WO 90/15070 (1990-12-01), None
patent: WO 91/06678 (1991-05-01), None
patent: WO 91/13075 (1991-09-01), None
patent: WO 92/10587 (1992-06-01), None
patent: WO 92/10588 (1992-06-01), None
patent: WO 92/15712 (1992-09-01), None
patent: WO 92/16657 (1992-10-01), None
patent: WO 93/05183 (1993-03-01), None
patent: WO 93/13220 (1993-07-01), None
patent: WO 93/17126 (1993-09-01), None
patent: WO 93/21340 (1993-10-01), None
patent: WO 95/15970 (1995-06-01), None
Sokolov, B. P., “Primer extension technique for the detection of single nucleotide in genomic DNA,”Nucleic Acids Research, 18(12) :3671 (1990).
Delius H. et al., “Separation of complementary strands of plasmid DNA using the biotin-avidin system and its application to heteroduplex formation and RNA/DNA hybridization in electron microscopy,”Nucleic Acid Research, 13(15) : 5457-5469 (1985).
Kuppuswamy, M. N. et al., “Single nucleotide primer extension to detect genetic diseases: Experimental application to hemophilia B (factor IX) and cystic fibrosis genes,”Proc. Natl. Acad. Sci. USA, 88:1143-1147 (1991).
Nyren', P. et al., “Solid Phase DNA Minisequencing by an Enzymatic Luminometric Inorganic Pyrophosphate Detection Assay,”Anal. Biochemistry, 208:171-175 (1993).
Cotton, R.G.H., “Current methods of mutation detection,”Mutation Research, 285:125-144 (1993).
Kornher J. S. et al., “Mutation Detection Using Nucleotide Analogs that alter electrophoretic Mobility,”Nucleic Acids Research, 17 : (19) 7779-7784 (1989).
Lee, Jin-Sung et al., “Identification of the most common mutation within the porphobilinogen deaminase gene in Swedish patients with acute intermittent porphyria,”Proc. Natl. Acad. Sci. USA, 88:10912-10915 (1991).
Chemical Abstracts,113 (25) : 161 (1990) , Edwards, A. et al., “Automated DNA sequencing of the human HPRT locus,”Genomics, 6(4) :593-608 (1990).
Chemical Abstracts, 117 (23) :645 (1992) , Monnat, R.J. et al., “Nucleotide sequence analysis of human hypoxanthine phosphoribosyltransferase (HPRT) gene deletions,”Genomics, 13(3) :777-787 (1992).
Chemical Abstracts,104(25) :161 (1986) Kim, S. H. et al., “The organization of the human HPRT gene,”Nucleic Acids Res., 14(7) :3103-3118 (1986).
Khrapko, K.R. et al., “A method for DNA sequencing by hybridazaiton with oligonucleotide matrix,”DNA Sequence-DNA Sequencing and Mapping, 1(3) :375-388 (1991).
Kharpko, K.R. et al., “An oligonucleotide hybridization approach to DNA sequencing,”FEBS Ltrs., 256(1,2) :188-122 (1989).
Barinaga, M., “Will ‘DNA Chip’ Speed Genome Initiative,”Science, pp. 253 (1991).
Kostichka, A. J., “High Speed Automated DNA Sequencing In Ultrathin Slab Gels,”Biotechnology, 10:78-81 (1992).
Krook, Anna, et al., “Rapid and simultaneous detection of multiple mutations by pooled and multiplex single nucleotide primer extension: application to the study of insulin-responsive glucose transporter and insulin receptor mutations in non-insulin-dependent diabetes,”Hum. Mol. Genet.. 1(6): 391-395 (1992).
Caskey C. Thomas
Metspalu Andres
Shumaker John
Baylor College of Medicine
Fredman Jeffrey
Hamilton Brook Smith & Reynolds P.C.
Pharmacia Biotech AB
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