Parallel chromosomal stacking of traits in bacteria

Details Parallel chromosomal stacking of traits in bacteria Parallel chromosomal stacking of traits in bacteria

2007-08-07

2007-08-07

Vogel, Nancy (Department: 1636)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S471000, C435S472000

Reexamination Certificate

active

10734778

ABSTRACT:
The invention describes a method for the stacking of traits in a recombination proficient host using a phage transduction system. The method makes use of a nucleic acid integration cassette that has homology to a specific site on a host chromosome for the insertion of genetic elements and the stacking of traits. Repetition of the method results in the stacking of traits on a single genetic element.

REFERENCES:
patent: 6287862 (2001-09-01), DelCardayre et al.
patent: 6534315 (2003-03-01), Bauer et al.
patent: WO 01/18222 (2001-03-01), None
patent: WO 03/089605 (2003-10-01), None
Datsenko, Kirill A. et al., One-step inactivation of chromosomal genes inEscherichia coliK-12 using PCR Products, Proceedings of the National Academy of Sciences of USA, 2000, pp. 6640-6645, vol. 97, No. 12., Washington, DC.
Zhou, Shengde et al., Production of Optically Pure D-Lactic Acid in Mineral Salts Medium by Metabolically EngineeredEscherichia coliW3110, Applied Environmental Microbiology, Jan. 2003, pp. 399-407, vol. 69, No. 1, American Society for Microbiology.
Yuan, Luke Z. et al., Chromosomal promoter replacement of the isoprenoid pathway for enhancing carotenoid production ofE. coli, Metabolic Engineering, Jan. 2006, pp. 79-90, vol. 8, Elsevier Inc.
Perdelchuk, M.Y. et al., Gene, vol. 187, pp. 231-238, 1997.

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