Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Reexamination Certificate
2000-05-25
2002-04-09
Salimi, Ali R. (Department: 1648)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
C435S069100, C435S091100, C435S091330, C530S350000, C530S403000, C536S023720
Reexamination Certificate
active
06368832
ABSTRACT:
TABLE OF CONTENTS
I. FIELD OF THE INVENTION
II. BACKGROUND OF THE INVENTION
III. SUMMARY OF THE INVENTION
IV. BRIEF DESCRIPTION OF THE DRAWINGS
V. DETAILED DESCRIPTION OF THE INVENTION
VI. EXAMPLES
A. Example 1: Identification of the Papilloma Virus Genome VS19-6-G
B. Example 2: Cloning of the Papilloma Virus Genome VS19-6-G
WHAT IS CLAIMED:
ABSTRACT
This is a national phase filing of the application Ser. No. PCT/DE96/01369, which was filed with the Patent Corporation Treaty on Jul. 19, 1996, and is entitled to priority of the German Patent Application P 195 26 386.3, filed Jul. 19, 1995.
I. FIELD OF THE INTENTION
This invention relates to a DNA coding for a peptide of a papilloma virus major capsid protein. Moreover, this invention deals with a papilloma virus genome containing such a DNA. In addition, this invention concerns proteins coded by the papilloma virus genome and virus-like particles as well as antibodies directed against them and their use for diagnosis, treatment and vaccination.
II. BACKGROUND OF THE INVENTION
It is known that papilloma viruses infect the epithelium of human beings and animals. Human papilloma viruses (hereinafter referred to as HP viruses) are found in benign epithelial neoplasms, e.g. warts, condylomas in the genital zone, and malignant epithelial neoplasms, e.g. carcinomas of the skin and the uterus. Zur Hausen, 1989
, Cancer Research
49:4677-4681. HP viruses are also considered for the growth of malignant tumors in the respiratory tract. Zur Hausen, 1976
, Cancer Research
36:530. Besides, HP viruses are considered at least jointly responsible for the growth of squamous carcinomas of the lung. Syrjanen, 1980
, Lung
158:131-142.
Papilloma viruses have an icosahedral capsid without envelope in which a circular, double-stranded DNA molecule of about 7900 bp is present. The capsid comprises a major capsid protein (L1) and a minor capsid protein (L2). Both proteins, coexpressed or L1 expressed alone, result in vitro in the formation of virus-like particles Kimbauer et al., 1993
, Journal of Virology
67:6929-6936.
Papilloma viruses cannot be proliferated in monolayer cell culture. Therefore, their characterization is extremely difficult, the detection of papilloma viruses already creating considerable problems. This applies especially to papilloma viruses in carcinomas of the skin. A reliable detection thereof and thus well-calculated steps taken thereagainst are not possible by now.
Thus, it is the object of the present invention to provide a product by which papilloma viruses can be detected, particularly in carcinomas of the skin. Furthermore, a product should be provided to be able to take therapeutic steps against these papilloma viruses.
III. SUMMARY OF THIS INVENTION
This invention relates to a DNA coding for a peptide of a papilloma virus major capsid protein. Moreover, this invention deals with a papilloma virus genome containing such a DNA. Furthermore, this invention concerns proteins coded by the papilloma virus genome and virus-like particles as well as antibodies directed thereagainst and the use thereof for diagnosis, treatment and vaccination.
REFERENCES:
patent: A44 15 743 (1995-11-01), None
patent: A0 301 289 (1989-02-01), None
patent: A0 370 625 (1990-05-01), None
patent: WOA94 05792 (1994-03-01), None
Browne et al., 1988, “Analysis of the L1 Gene Product of Human Papillomavirus Type 16 by Expression in a Vaccinia Virus Recombinant,”J. Gen. Virology 69: 1263-1273.
Egawa et al., 1993, “Two Novel Types of Human Papillomavirus, HPV 63 and HPV 65: Comparisons of their Clinical and Histological Features and DNA Sequences to Other HPV Types,”Virology 194: 789-799.
Hagensee et al., 1993, “Self-Assembly of Human Papillomavirus Type 1 Capsids by Expression of the L1 Protein Alone or by Coexpression of the L1 and L2 Capsid Proteins,”Journal of Virology 67: 315-322.
Jarrett et al., 1991, “Studies on Vaccination Against Papillomaviruses: Prophylactic and Therapeutic Vaccination with Recombinant Structural Protein,”Virology 184:33-42.
Kirnbauer et al., 1993, “Efficient Self-Assembly of Human Papillomavirus Type 16 L1 and L1-L2 into Virus-Like Particles,”Journal of Virology 67: 6929-6936.
Shamanin et al., 1994, “Specific Types of Human Papillomavirus Found in Benign Proliferations and Carcinomas of the Skin in Immunosuppressed Patients,”Cancer Research 54: 4610-4613.
Shamanin et al., 1996, “Human Papillomavirus Infections in Nonmelanoma Skin Cancer from Renal Transplant Recipients and Nonimmunosuppressed Patients,”Journal of the National Cancer Institute 88(12):802-811.
Syrjänen, K.J., 1980, “Bronchial Squamous Cell Carcinomas Associated with Epithelial Changes Identical to Condylomatous Lesions of the Uterine Cervix,”Lung 158:131-142.
zur Hausen, H., 1976, “Biochemical Approaches to Detection of Epstein-Barr Virus in Human Tumors,”Cancer Research 36: 678-680.
zur Hausen, H., 1976, “Condylomata Acuminata and Human Genital Cancer,”Cancer Research 36:794.
zur Hausen, H., 1989, “Papillomaviruses in Anogenital Cancer as a Model to Understand the Role of Viruses in Human Cancers,”Cancer Research 49: 4677-4681.
Xi et al., J. of Gen. Virology, 1991, vol. 72, pp. 2981-2988 (abstract only).
Zhou et al., Virology, 1991, vol. 185, pp. 251-257.
Tomita et al., J. of Virology, 1987, vol. 61, pp. 2389-2394.
Rose et al., J. of Virology, 1993, vol. 67, pp. 1936-1944.
De Villiers-Zur Hausen Ethel-Michele
Hausen Harald Zur
Shamanin Vladimir
Deutsches Krebsforschungszentrum Stiftung des Öffentlichen Recht
Pennie & Edmonds LLP
Salimi Ali R.
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