Papilloma pseudo-virus and preparation

Chemistry: molecular biology and microbiology – Vector – per se

Reexamination Certificate

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C435S235100, C435S239000

Reexamination Certificate

active

06878541

ABSTRACT:
The invention involves a papilloma pseudo-virus that can induce immune response after oral intake as well as its preparation. It is characterized in that HPV or BPV pseudo-virus are made by disrupting HPV-VLP or BPV-VLP, mixing them with plasmids (plasmids or DNA vaccine), and reassembling them into the pseudo-viruses (VLPs with plasmids inside). Oral administration of the pseudo-viruses will result in delivery to mucosal and systemic lymphoid tissues and induce immune responses for disease prevention and treatment. The pseudo-virus induces stronger immune response than DNA vaccines. Additionally, the pseudo-virus can be applied in gene therapy by bringing the therapeutic genes into lymphoid tissues in the human body.

REFERENCES:
patent: 6420160 (2002-07-01), Bloch
patent: WO 9802548 (1998-01-01), None
patent: WO 9913056 (1999-03-01), None
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Shi, et al “Papillomavirus Pseudovirions as a Novel Mucosal Vaccine,” HPV 2000 Barcelona, Immunology/HIV/Vaccines: Poster Session 3, Abstract No. 448.
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Shi, et al “Papillomavirus Pseudovirus: a Novel Vaccine To Induce Mucosal and Systemic Cytotoxic T-Lymphocyte Responses,” Journal of Virology, Nov. 2001, vol. 75, No. 21, pp. 10139-10148.

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