Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide confers pathogen or pest resistance
Patent
1998-01-09
2000-10-24
McElwain, Elizabeth
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide confers pathogen or pest resistance
A01H 100
Patent
active
061370304
ABSTRACT:
Disclosed are PAP mutants having reduced phytotoxicity compared to wild-type PAP, and which confer broad spectrum resistance to viruses and/or fungi in plants. One group of PAP mutants is characterized by at least one amino acid substitution in the N-terminus of mature PAP, such as the Glycine 75 residue or the Glutamic acid 97 residue; two groups of additional PAP mutants are characterized by truncations in the N-terminal region of mature PAP and truncations or amino acid substitutions in the C-terminal region of mature PAP, respectively; and a further group are enzymatically inactive which still exhibit anti-fungal properties. Also disclosed are DNA molecules encoding the PAP mutants, mutant PAP DNA constructs, and transgenic seed and plants containing the DNAs. Further disclosed are methods for identifying PAP mutants having reduced phytotoxicity, as well as isolated and purified PAP mutants identified by the method.
REFERENCES:
patent: 5304730 (1994-04-01), Lawson et al.
Abel et al., Science 232:738-43 (1986).
Cuozzo et al., Bio/Technology 6:549-57 (1988).
Hemenway et al., EMBO J. 7:1273-80 (1988).
Stark et al., Bio/Technology 7:1257-62 (1989).
Lawson et al., Bio/Technology 8:127-34 (1990).
Kawchuk et al., Mol. Plant-Microbe Interactions 3(5):301-07 (1990).
Lodge et al., Proc. Natl. Acad. Sci. USA 90:7089-93 (1993).
Irvin et al., Pharmac. Ther. 55:279-302 (1992).
Endo et al., Biophys. Res. Comm., 150:1032-36 (1988).
Hartley et al., FEBS Lett. 290:65-68 (1991).
Beachy et al., Ann. Rev. Phytopathol. 28:451-74 (1990).
Golemboski et al., Proc. Natl. Acad. Sci. USA 87:6311-15 (1990).
Hayashi et al., J. Bioenerg. Biomem. 22:451-71 (1990).
Dore et al., Nuc. Acids Res. 21(18):4200-05 (1993).
Monzingo et al., J. Mol. Biol. 233:705-15 (1993).
Chen et al., Plant Pathol. 40:612-20 (1991).
McElwain Elizabeth
The State University of Rutgers
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