Panel of biomarkers for peripheral arterial disease

Chemistry: analytical and immunological testing – Peptide – protein or amino acid

Reexamination Certificate

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C436S063000, C702S019000, C702S001000

Reexamination Certificate

active

07998743

ABSTRACT:
The invention provides biomarkers including β-2-microglobulin, Cystatin C, hsCRP and glucose as well as methods for using the biomarkers for diagnosing and/or assessing the risk of peripheral artery disease in a subject. In some embodiments, the subject being tested may be suffering from or at risk of other circulatory diseases, including coronary artery disease. Hemoglobin A1c or other proxies for measuring glucose levels may be substituted for or measured in addition to glucose in the context of the present invention.

REFERENCES:
patent: 2004/0121343 (2004-06-01), Buechler et al.
patent: 1 615 036 (2006-01-01), None
O'Hare, A.M., et al.; “Cystatin C and incident peripheral arterial disease events in the elderly,” 2005,Arch. Intern. Med., vol. 165, pp. 2666-2670.
Saijo, Y., et al.; “Relationships of β2-microglobulin to arterial stiffness in Japanese subjects,” 2005,Hypertens. Res., vol. 28, No. 6, pp. 505-511.
Thongboonkerd, V., et al.; “Proteomic analysis reveals alterations in the renal Kallikrein pathway during hypoxia-induced hypertension,” 2002,J. Biol. Chem., vol. 277, No. 33, pp. 34708-34716.
Nedelkov, Dobrin, et al., “Design and use of multi-affinity surfaces in biomolecular interaction analysis-mass spectrometry (BIA/MS): a step toward the design of SPR/MS arrays,”Journal of Molecular Recognition, vol. 16, pp. 15-19 (2003).
Schillinger, Martin, et al., “Joint Effects of C-Reactive Protein and Glycated Hemoglobin in Predicting Future Cardiovascular Events of Patients With Advanced Atherosclerosis,”Circulation AHA Journals, pp. 2323-2328 (Nov. 11, 2003).
Standl, E., et al., “Predictors of 10-year macrovascular and overall mortality in patients with NIDDM: the Munich General Practioner Project,”Diabetologia, vol. 39, pp. 1540-1545, (1996).

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