Panel cell used for granulocyte antibody detection

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C435S455000

Reexamination Certificate

active

08008028

ABSTRACT:
A panel cell for detecting anti-HNA antibody is disclosed. The panel cell is obtained by introducing a DNA coding for an HNA antigen corresponding to the anti-HNA antibody into a cell so as to enable the expression of the DNA under the condition for use in the detection procedure, wherein the cell for DNA introduction exhibits no detectable reaction with anti-HLA-ABC antibody, anti-HLA-DR antibody, anti-HLA-DQ antibody, anti-HLA-DP antibody, anti-HNA-1 antibody, anti-HNA-2a antibody, anti-HNA-3a antibody, anti-HNA-4 antibody, anti-HNA-5 antibody, and serum from normal subject, in the detection procedure. The panel cell allows accurate and rapid detection of granulocyte antibody.

REFERENCES:
patent: 2005/0003484 (2005-01-01), Hirano et al.
Lucas et al., Transfusion, 2002, v.42, pp. 462-468.
Bux et al., Blood,1999v.93, p. 357-362.
K. P. M. Van Kessel et al., “Quantitation of Conjugate Formation Between Human Polymorphonuclear Leukocytes and Antibody-Coated Target Cells by Flow Cytometry: The Role of Fc Receptor and LFA-1 Antigen”, Journal of Leukocyte Biology, vol. 46, No. 5, pp. 467-475, 1989.
K. Miyazaki et al., “P57-O: Preparation of HNA-1a and 1b Panels by Using FCGR3B Gene Expressing Cell,” Japanese Journal of Transfusion Medicine, vol. 50, No. 2, pp. 297, P57-O, 2004 (English translation).
K. Yasui et al., “Detection of Anti-HNA Antibody in Serum With HNA-1a, -1b, and -2a Gene Expression Panel Cell Line,” Ketsueki Jigyo, vol. 29, No. 2, p. 293, 42, Aug. 2006 (English translation).
F. Hirayama et al., “New Detection System of Granulocyte Antibodies”, Ketsueki Jigyo, vol. 29, No. 2, p. 251, 3-3, Aug. 2006 (English translation).
K. Yasui et al., “O-51: Production of HNA-1a and -1b Gene Expressing Panel Cell Lines for Detecting Granulocyte Antibody,” Japanese Journal of Transfusion Medicine, vol. 52, No. 2, p. 252, O-51, May 2006 (English translation).

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