Pancreas elastase 1-specific antibody, a process for obtaining i

Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Animal cell – per se – expressing immunoglobulin – antibody – or...

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5303881, 53038826, 5303891, 435975, C12N 500, C07K 1640, G01N 3353

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active

056228376

ABSTRACT:
A process is described for obtaining highly specific pancreas elastase 1 antibodies which react both with bodily fluids and with stools. Such an antibody is obtainable by immunizing with an antigen having the amino acid sequence Thr-Met-Val-Ala-Gly-Gly-Asp-Ile-Arg (SEQ ID NO:1) or immunologically active partial peptides thereof. A test kit containing such antibodies is suitable for the diagnosis and course monitoring of chronic and acute pancreatitis as well as mucoviscidosis in bodily fluids and/or in stools.

REFERENCES:
Wendorf et al. FEBS Lett., 1989, 249:275, Localization . . . elastapel.
Sziegoleit et al., Clin. Biochem., 1989, 22:85, Elastasel . . . Peces.
Macierer et al., Meth. Enzymol., 70:49, 1980, Proteins . . . Antigens.
Tani et al., J. Biol. Chem., 263:1231, 1988, Identification . . . Cloning.
Murata et al., Enzyme, 30:29, 1983, Radioimmunoassay . . . elastasel.
Sziegoleit et al., Biol Chem Hoppe Seyler, 368:1613, 1987.
Chem Abstracts, vol. 110 p. 284, 1989, Monoclonal . . . diagnosis, Kurata al.
Chem. Abstracts, vol. 114, p. 97133, 1991, Enzyme . . . elastase, Hayakawa et al.

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