Paenibacillus naphthalenovorans that degrades naphthalene

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor

Reexamination Certificate

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C435S252400, C435S252500, C435S262500, C424S093460, C424S093300

Reexamination Certificate

active

06905864

ABSTRACT:
Polyaromatic hydrocarbons are found in contaminated soils and groundwater and are a large class of compounds for which treatment by bioremediation is sought. Accordingly, indigenous organisms that can degrade polyaromatic hydrocarbon are needed. Presented are strains of thePaenibacillusgenus that degrade low molecule weight polyaromatic, and, in the presence of phenanthrene, high molecular weight polyaromatic hydrocarbons. These bacteria strains are unique among PAH-degrading bacterial strains because they form endospores and therefore can survive adverse environmental conditions. Among the strains presented are several from “P. naphthalenovorans”, a newly described species. Also presented are compositions for bioremediation comprising the bacterial strains of the invention, and a method for isolating the strains and a method for using the strains for bioremediation.

REFERENCES:
Meyer et al., Microbiology (Reading, United Kingdom) (Jul. 1999), 145(7), 1731-1741.
Ash et al., “Molecular Identification of rRNA Group 3 Bacilli(Ash, Farrow, Wallbanks and Collins) Using a PCR Probe Test”, 1993 Antonie van Leewenhoek 64:253-260.
Pichinoty et al., “Bacillus gordonaesp. nov., une Nouvelle Espéce Appartenant au Second Groupe Morphologique, Dégradant Divers Composés Aromatiques”, Ann. Inst. Pasteur/Microbiol. 1986 137A:65-78.
Heydrickx et al.,Paenibacillus(FormerlyBacillus)gordonae(Pichinotiv et al. 1986) Ash et al. 1994 is a Later Subjective Synonum ofPaenibacillus(FormerlyBacillus)validus(Nakamura 1984) Ash et al., 1994:Emended Descripton of P. validus. International Journal of Systemic Bacteriology 45:661-669.

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