Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1996-06-14
1999-06-15
Jones, W. Gary
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
536 231, 536 243, 935 76, 935 77, 935 78, C12Q 168, C07K 2102, C07K 2104, C12N 1500
Patent
active
059121246
ABSTRACT:
The present invention relates to the detection of a polynucleotide using a padlock probe, whereby a hybridization process is combined with ligation to irreversibly capture polynucleotides of interest. The invention relates both to the padlock probe itself, as well as to methods of use, which allow for varying levels of sensitivity due to amplification of signal processes built into the system. Additionally, preferred formats are disclosed for use of the padlock probes, including the use of microfluidics.
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Burke William J.
Jones W. Gary
Sarnoff Corporation
Whisenant Ethan
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