Packings combining protein to a support via a spacer

Details Packings combining protein to a support via a spacer Packings combining protein to a support via a spacer

1994-08-22

1996-01-02

Therkorn, Ernest G.

Liquid purification or separation

With means to add treating material

Chromatography

2105021, 210635, 210656, 502403, B01D 1508

Patent

active

054805422

ABSTRACT:
A packing for chromatography whereby a low molecular weight substance contained in biological samples can be analyzed directly at a high accuracy without any pretreatments. This packing is combining a protein such as avidin and ovomucoid to the support via a straight or branched spacer optionally having a functional group such as a cyano group. Enantiomers interact differentially with proteins, and thereby optically separated. Further, a purification column packed with said packing is disclosed. According to the present invention, a low molecular weight substance can be directly and accurately analyzed without requiring any pretreatment for removing biological macromolecules. When it is employed in the purification column, the time required for the pretreatment can be shortened.

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