Packing material and cartridge for solid phase extraction

Details Packing material and cartridge for solid phase extraction Packing material and cartridge for solid phase extraction

2001-08-21

2003-08-05

Pezzuto, Helen L. (Department: 1713)

Synthetic resins or natural rubbers -- part of the class 520 ser

Synthetic resins

Cellular products or processes of preparing a cellular...

C521S056000, C521S060000, C526S149000, C526S303100, C526S307100, C526S307700, C526S323100, C526S323200, C526S336000

Reexamination Certificate

active

06602928

ABSTRACT:

FIELD OF THE INVENTION
The present invention relates to a packing material for solid phase extraction, which has a specific exclusion limit molecular weight or a specific pore size; a column and a cartridge for solid phase extraction, each using the packaging material; and a process for treating a medical sample, which uses the column or cartridge.
BACKGROUND OF THE INVENTION
In many cases, a liquid-liquid extraction method has been heretofore used for extracting a sample from a liquid, however, this method has a problem in that the operation is cumbersome, a solvent is used in a large amount and the solvent used greatly affects the environment and the human body. In recent years, with the progress of synthesis methods, a solid phase extraction method using silica-type or synthetic polymer-type porous particles capable of treating a large amount of sample through a simple operation using a small amount of solvent by virtue of automation has been used.
The packing material for use in the solid phase extraction is an inorganic substrate such as silica gel or chemical bonding-type silica gel obtained by chemically modifying the surface of silica gel, or an organic substrate such as a synthetic polymer-type substrate represented by polystyrene-divinylbenzene or a substrate obtained by chemically modifying the surface thereof.
Heretofore, inorganic or organic packing materials are known to be used in the solid phase extraction. For example, JP-A-6-258203 (the term “JP-A” as used herein means an “unexamined published Japanese patent application”) describes a cartridge column for solid phase extraction, which is packed with a copolymer of divinylbenzene and polyhydric alcohol poly(meth)acrylic acid ester. However, in this patent publication, only the hydrophobicity and hydrophilicity on the surface of the crosslinked copolymer particle are discussed.
As such, only the chemical properties, such as hydrophobicity or hydrophilicity on the particle surface, of the packing material for solid phase extraction has been studied, but studies on the physical properties such as pore size distribution have not been satisfactorily made.
SUMMARY OF THE INVENTION
An object of the present invention is to obtain a packing material having excellent properties based on the physical properties such as pore size range of the packing material while taking account of the chemical properties such as hydrophobicity or hydrophilicity on the particle surface of conventional packing materials for solid phase extraction.
As a result of extensive investigations to overcome the above-described problems, the present inventors have found that a packing material for solid phase extraction, which ensures excellent recovery of a sample can be obtained by controlling the pore size distribution. The present invention has been accomplished based on this finding.
More specifically, the present invention provides to the following embodiments.
[1] A packing material for solid phase extraction, comprising particles having an exclusion limit molecular weight of from 1×10
3
to 1.5×10
4
in gel permeation chromatography using a standard polystyrene as the measuring sample.
[2] The packing material for solid phase extraction as described in [1] above, wherein the particle is a synthetic polymer particle obtained by polymerizing monomers containing at least a crosslinkable monomer.
[3] The packing material for solid phase extraction as described in [2] above, wherein the synthetic polymer particle is a polymer of at least a crosslinkable monomer (A) and a non-crosslinkable monomer (B).
[4 The packing material for solid phase extraction as described in [3] above, which contains an aromatic divinyl compound as the crosslinkable monomer (A) in an amount of 30% by mass or more based on the total amount of monomers.
[5] The packing material for solid phase extraction as described in [3] or [4] above, which contains a polyhydric alcohol poly(meth)acrylic acid ester as the crosslinkable monomer (A) in an amount of 10% by mass or more based on the total amount of monomers.
[6] The packing material for solid phase extraction as described in any one of [3] to [5] above, which contains an N-vinylcarboxylic acid amide as the non-crosslinkable monomer (B) in an amount of 5 to 60% by mass based on the total amount of monomers.
[7] The packing material for solid phase extraction as described in [6] above, wherein the N-vinylcarboxylic acid amide is N-vinylacetamide.
[8] The packing material for solid phase extraction as described in any one of [1] to [7] above, which is packed into a column, a cartridge or a reservoir on use.
[9] The packing material for solid phase extraction as described in any one of [1] to [8] above, which is used for concentrating an objective component and/or removing impurities or contaminants.
[10] The packing material for solid phase extraction as described in any one of [1] to [9] above, which has an average particle size of 1 to 200 &mgr;m.
[11] A column for solid phase extraction] above, which is packed with the packing material for solid phase extraction described in any one of [1] to [10] above.
[12] A cartridge for solid phase extraction] above, which is packed with the packing material for solid phase extraction described in any one of [1] to [10].
[13] The column for solid phase extraction as described in [11] above, which is used for concentrating an objective component and/or removing impurities or contaminants.
[14] The cartridge for solid phase extraction as described in [12] above, which is used for concentrating an objective component and/or removing impurities or contaminants.
[15] A process for treating an environment- or medical sample, which uses the column for solid phase extraction described in [11] or [13] above.
[16] A process for treating an environment- or medical sample, which uses the cartridge for solid phase extraction described in [12] or [14] above.
[17] The process for treating an environment- or medical sample as described in [15] or [16] above, which is used for the treatment of a protein component-containing sample.
DESCRIPTION OF THE INVENTION
The packing material for solid phase extraction of the present invention is a particle having an exclusion limit molecular weight of from 1×10
3
to 1.5×10
4
, preferably 7×10
3
to 1.5×10
4
, in gel permeation chromatography using a standard polystyrene as the measuring sample. The surface area of the particles is 350 to 800 m
2
/g, preferably 450 to 700 m
2
/g and more preferably 550 to 670 m
2
/g . This particle may use an inorganic substrate or an organic substrate, however, a synthetic polymer-type packing material for solid phase extraction using an organic substrate is preferred when taking into account the easiness of bulk synthesis, good reproduction in the control of pore size and the like. Examples of the synthetic polymer using an organic substrate include polystyrene-divinylbenzene type; poly(meth)acrylate type such as polyacrylate, glycidyl methacrylate and ethylene glycol dimethacrylate, and various copolymers thereof.
In the present invention, the packing material for solid phase extraction is preferably obtained by polymerizing monomers containing at least a crosslinkable monomer (A) or by copolymerizing a crosslinkable monomer (A) and a non-crosslinkable monomer (B). The packing material for solid phase extraction of the present invention includes polymers obtained by polymerizing only the crosslinkable monomer (A) as the monomer without containing the non-crosslinkable monomer (B). The crosslinkable monomer (A) for use in the present invention is not particularly

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