Packaging systems for human recombinant adenovirus to be used in

Details Packaging systems for human recombinant adenovirus to be used in Packaging systems for human recombinant adenovirus to be used in

1997-07-15

2000-03-07

Campell, Bruce R.

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

514 44, 424 9321, 536 231, 435 691, 4353201, 435455, C12N 1500

Patent

active

06033908&

ABSTRACT:
The invention provides improved methods and products based on adenoviral materials which can advantageously be used in for instance gene therapy. In one aspect an adenoviral vector is provided which has no overlap with a suitable packaging cell line which is another aspect of invention. This combination excludes the possibility of homologous recombination, thereby excluding the possibility of the formation of replication competent adenovirus. In another aspect an adenovirus based helper construct which by its size is incapable of being encapsidated. This helper virus can be transferred into any suitable host cell making it a packaging cell. Further a number of useful mutations to adenoviral based materials and combinations of such mutations are disclosed, which all have in common the safety of the methods and the products, in particular avoiding the production of replication competent adenovirus and/or interference with the immune system. Further a method of intracellular amplification is provided.

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