Packaging cells

Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Primate cell – per se

Reexamination Certificate

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Details

C435S325000, C435S455000, C435S320100

Reexamination Certificate

active

07070994

ABSTRACT:
The invention described herein allows the production of recombinant retroviruses (retroviral vector particles) from producer cells which are safer and of higher titer than normal. In addition, methods are provided for making helper cells which, when a recombinant retrovirus genome is introduced to make a producer line, produce particles that are targeted toward particular cell types. Methods are also provided for making recombinant retrovirus systems adapted to infect a particular cell type, such as a tumor, by binding the retrovirus or recombinant retrovirus in the particular cell type. Methods are also provided for producing recombinant retroviruses which integrate in a specific small number of places in the host genome, and for producing recombinant retroviruses from transgenic animals.

REFERENCES:
patent: 5591624 (1997-01-01), Barber et al.
patent: 5817491 (1998-10-01), Yee et al.
patent: 6165715 (2000-12-01), Collins et al.
patent: WO 9708330 (1997-03-01), None
Ban, Josef et al . “Bovine Leukemia Virus Packaging Cell Line for Retrovirus-mediated Gene Transfer.” Journal of General Virology (1989) vol. 70: 1987-1993.
O'Neill, Raymond R. et al. “Envelope and Long Terminal Repeat Sequences of a Cloned Infectious NZB Xenotropic Murine Leukemia Virus.” Journal of Virology (Jan. 1985) vol. 53, No. 1: 100-106.
Cosset, Francois-Loic et al. “A New Avian Leukosis Virus-Based Packaging Cell Line That Uses Two Separate Transcomplementing Helper Genomes.” Journal of Virology (Mar. 1990) vol. 64, No. 3: 1070-1078.

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