Packaging cell

Details Packaging cell Packaging cell

2006-02-07

2006-02-07

Housel, James (Department: 1648)

Chemistry: molecular biology and microbiology

Vector, per se

C435S325000, C435S235100, C435S456000, C435S069100

Reexamination Certificate

active

06995009

ABSTRACT:
A virus-producing cell sustaining the ability to produce viruses at high titer is successfully constructed by expressing the virus structural gene under the regulation of EF1α promoter. In this virus-producing cell, the virus structural gene is ligated to a selection marker gene via IRES and domains other than the protein coding domain are eliminated from the DNA encoding virus structural proteins. Thus, reduction of the titer due to cell passages can be prevented and emergence of wild type viruses caused by unfavorable recombination of the virus genome can be inhibited.

REFERENCES:
patent: 2002/0123146 (2002-09-01), Klatzmann et al.
patent: WO 98/02529 (1998-01-01), None
patent: WO 99/64568 (1999-12-01), None
Hobbs et al., Biochem Biophys Res Comm 199 vol. 252, pp. 368-372.
Duisit et al., “Functional Characterization of Adenoviral/Retroviral Chimeric Vectors and Their Use for Efficient Screening of Retroviral Producer Cell Lines,”Human Gene Therapy,10:189-200 (Jan. 20, 1999).
Kitamura et al.,Jikken Igaku Bessatsu, Shin Idenshi kouguku Handbook,pp. 245-9 (1998).
Kitamura et al., “New experimental approaches in retrovirus-mediated expression screening”,International Journal of Hematology,67 (1998), pp. 351-359.
Morita et al., “Plat-E: an efficient and stable system for transient packaging of retroviruses”,Gene Therapy, (2000) 7:1063-1066.
Pear et al., “Production of high-titer helper-free retroviruses by transient transfection”,Proc. Natl. Acad. Sci. USA,vol. 90, pp. 8392-8396.
Yogalingam et al., “Regulation of N-Acetylgalactosamine 4-Sulfatase Expression in Retrovirus-Transduced Feline Mucopolysaccharidosis Type VI Muscle Cells”,DNA and Cell Biology, vol. 18, No. 3 (1999), pp. 187-195.
A tutorial of Phoenix Ecotropic and Amphotropic Packaing Lines.
A tutorial of Improvements to retroviral producer lines; Phoenix Systems.
Hobbs et al., “Development of a bicistronic vector driven by the human polypeptide chain elongation factor lalpha promoter for creation of stable mammalian cell lines that express very high levels of recombinant proteins,”Biochemical and Biophysical Research Communications, vol. 252, No. 2, Nov. 18, 1998, pp. 368-372.
Rigg et al., “A Novel Human Amphotropic Packaging Cell Line: High Tilter, Complement Resistance, and Improved Safety”Virology,vol. 218, No. 1, 1996, pp. 290-295.
Swift et al., “Rapid production of retroviruses for efficient gene delivery to mammalian cells using 293T cell-based systems,”Current Protocols in Immunology, Supp. 31, 1999, pp. 10.17.14-10.17.29.
Yang et al., “Generation of Retroviral Vector for Clinical Studies Using Transient Transfection,”Human Gene Therapy, vol. 10, No. 1, Jan. 1, 1999, pp. 123-132.

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