P450 oxygenases and methods of use

Details P450 oxygenases and methods of use P450 oxygenases and methods of use

2008-07-22

2008-07-22

Pak, Yong D (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

C435S004000, C435S006120, C435S025000, C435S069100, C435S071100, C435S132000, C435S252300, C435S320100, C435S440000, C536S023200

Reexamination Certificate

active

07402417

ABSTRACT:
This disclosure provides the nucleic acid and protein sequences of novel P450 oxygenases that utilize, at least, taxoid substrates, including taxadiene isomers. The disclosed oxygenases, such as a taxoid 5α-hydroxylase, hydroxylate, at least, the C5 position of a taxoid. Also provided are methods of introducing oxygen at the C5 position of a taxoid and methods of producing the anti-cancer drug, paclitaxel (also known as Taxol™), and other taxoids, such as paclitaxel intermediates.

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