P gene promoter constructs for floral-tissue preferred gene expr

Chemistry: molecular biology and microbiology – Plant cell or cell line – per se ; composition thereof;... – Plant cell or cell line – per se – contains exogenous or...

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800205, 800250, 536 241, A01H 411, A01H 300, C07H 2104, C12N 510

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059553618

ABSTRACT:
This invention provides a transcriptional regulatory region of a gene which will be utilized to direct tissue-specific gene expression in plants such that a selective advantage is conferred upon said plants. The present invention relates to the isolation, characterization and utilization of a transcriptional regulatory region of a plant gene which is expressed in a floral tissue-specific manner. The transcriptional control region of said gene is demonstrated to drive gene expression in a floral-specific manner in vivo using transgenic plants.

REFERENCES:
An, G., Mitra, A., Choi, H.K., Costa, M.A., An, K., Thornburg, R.W., and Ryan, C.A. (1989). Functional analysis of the 3' control region of the potato wound-inducible proteinase inhibitor 11 gene. Plant Cell 1, 115-122.
An, W. and Wensink, P.C. (1995) Three protein binding sites from an enhancer geneser that regulates sex- and fat body-specific transcription of Drosophila yolk protein genes. The EMBO Journal 74 (6), 1221-1230.
Athma, P., Grotewold, E. and Peterson T. (1992). Insertional mutagenesis of the maize P gene by intragenic transpostion of Ac. Genetics 131:199-209.
Athma, P. and Peterson, T. (1991) Ac induces homologous recombination at the maize P locus. Genetics 128, 163-173.
Bingham, P.M. and Zachar, Z., (1989) Retrotransposon and the FB transposon from Drosophila melanogaster. In Berg, D. E. and Howe, M. M. (eds.) Mobile DNA, American Society for Microbiology. pp. 485-502.
Brink,R.A. and Nilan,R.A. (1952) The relation between light variegated and medium variegated pericarp in maize. Genetics 37:519-544.
Benfey, P.N., and Chua, N.-H. (1989) Regulated genes in transgenic plants. Science 244, 174-181.
Bowen, B. (1992). Anthocyanin genes as visual markers in transformed maize tissues. In Gallagher, S.R. (ed.) GUS Protocols: Using the GUS gene as a reporter of gene expression. Academic Press. San Diego, California. pp. 163-177.
Bureau T. E. and Wessler S. R. (1992) Tourist: A large family of small inverted repeat elements frequently associated with maize genes. The Plant Cell 4:1283-1294.
Carlson, D. P. and Ross, J. (1983) Human .beta.-globin promoter and coding sequences transcribed by RNA polymerase III. Cell 34:857-864.
Chen, J., Greenblatt, I.M., and Dellaporta, S.L. (1987) Transposition of Ac from the P locus of maize into unreplicated chromosomal sites. Genetics 117: 101-108.
Chen, J., Greenblatt, I.M., and Dellaporta, S.L. (1992) Molecular analysis of Ac transposition and DNA replication. Genetics 130:665-676.
Das. O.P., Messing, J. (1994) Variegated phenotype and developmental methylation changes of a maize allele originating from epimutation. Genetics 136:1121-1141.
De Block, M., Botterman, J., Vandewile, M., Docky, J., Thoen, C., Gossele, V., Rao Movva, N., Thompson, C., Van Montagu, M., and Leemans, J. (1987) Engineering herbicide resistance in plants by expression of a detoxifying enzyme. EMBO Journal 6:125-135.
Dennis, E., Gerlach, W., Pryor, A., Bennetzen, J., Inglis, A., Llewellyn, D., Sachs, M., Ferl, R. and Peacock, W. (1984) Molecular characterization of the maize Adhl gene. Nucl. Acids Res. 12: 3983-3990.
Emerson, R.A. (1917) Genetical studies of variegated pericarp in maize. Genetics 2:1-35.
Fromm, M. (1994) Production of transgenic maize plants via microprojectile-mediated gene transfer. In Freering, M. and Walbot, V. (eds), The Maize Handbook. Spring-Verlag, New York. pp. 677-684.
Gallie, D. R. and Young T.E. (1994) The regulation of gene expression in transformed maize aleurone and endosperm protoplasts. Plant Physiol. 106:929-939.
Gordon-Kamm, W. J., Spencer,M., Mangano, M.L., Adams, T.R., Daines, R.J., Start, W.G., O'Brien, J.V., Chambers, S.A., Adams, W.R. Jr., Willetts, N.G., Rice,T.B., Mackey, K.J., Krueger, R.W., Kausch, A.P. and Lemaux, P.G. (1990) Transformation of Maize Cells and Regeneration of Fertile Transgenic Plants. The Plant Cell 2:603-618.
Grotewold E., Drummond B.J., Bowen B., and Peterson T. (1995) The myb-homologous P gene controls phlobaphene pigmentation in maize floral organs by directly activating a flavonoid biosynthetic gene subset. Cell 76:543-553.
Grotewold, E., Athma, P., and Peterson, T. (1991) Alternatively spliced products of the maize P gene encode proteins with homology to the DNA binding domain of Myb-like transcription factors. Proc. Natl. Acad. Sci. USA 88:4587-4591.
Hull, M. W., Erickson, J., Johnston, M. and Engelke, D. R. (1994) tRNA genes as transcriptional repressor elements. Molecular and Cellular Biology 14:1266-1277.
Jefferson, R.A., Burgess, S.M. and Hirsh, D. (1986) .beta.-Glucuronidase from Escherichia coli as a gene fusion marker. Proc. Natl. Acad. Sci. USA 83:8447-8451.
Klein, T.M., Kornstein, L., Sanford, J.C., and Fromm, M.E. (1989) Genetic transformation of maize cells by particle bombardment. Plant Physiol. 91: 44044.
Konieczny, A., Voytas, D.F., Cummings, M.P. and Ausubel, F.M. (1991) A superfamily of Arabidopsis thaliana retrotransposons. Genetics 127 (4): 801-809.
Kyozuka, J., Olive,M., Peacock, W.J., Dennis, E.S. and Shimamoto K. (1994) Promotor Elements Required for Development Expression of the Maize Adhl gene in Transgenic Rice. The Plant Cell 6:799-810.
Lecheit, C., Peterson, T., Laired, A., Chen, J., Dellaporta, S., Dennis, E., Peacock, W.J., and Starlinger, P. (1989) Isolation and molecular analysis of the maize P locus. Mol. Gen. Genet. 219:225-234.
Ludwig, S.E., Bowen, B., Beach, L., and Wessier, S.R. (1990). A regulatory gene as a novel visible marker for maize transfornation. Science 246:449-450.
Lund G., Das O.P., and Messing J. (1995) Tissue-specific DNase I-sensitive sites of the maize P gene and their changes upon epimutation. The Plant Journal 7(5):797-807.
Moreno, M.A., Chen, J., Greenblatt, I., and Dellaporta, S.L. (1992) Reconstitutional mutagenesis of the maize P gene by short-range Ac transpostions. Genetics 131:939-956.
Peterson, T. (1990) Intragenic transpostion of Ac generates a new allele of the maize P gene. Genetics 126:469-476.
Radicella J.P., Brown D., Tolar L.A. and Chandler V.L. (1992) Allelic diversity of the maize B regulatory gene: different leader and promoter sequences of two B alleles determine distinct tissue specificities of anthocyanin production. Genes & Development 6:2152-2164.
Styles, E. D. and Ceska 0. (1977) The genetic control of flavonoid synthesis in maize. Can. J. Genet. Cytol. 19:289-302.
Styles, E. D. and Ceska 0. (1981) P and R control of flavonoids in Bronze coleptiles of maize. Can. J. Genet. Cytol. 23:691-704.
Su,T. Z. and El-Gewely, M.R. (1988) A multisite-directed mutagenesis procedure using T7 DNA polymerase: Application for reconstructing a mammalian gene. Gene 69:81-89.
Linger, E., Parsons, R.L.., Schmidt, R.J., Bowen, B., and Roth, B.A. (1993) Dominant negative mutants of Opaque2 suppress transactivation of a 22-IcD zein protein by opaque 2 in maize endosperm cells. Plant Cell 5: 831-841.
Voytas D. F., Cummings, M.P., Konieczny,A., Ausubel, F.M. and Rodermel, S.R. (1992) Copia-like retrotransposons are ubiquitous among plants. Proc. Natl. Acad. Sci. USA. 31: 7124-7128.
Walker E. L., Robbins,T. P., Bureau,T. E., Kermicle, J., and Dellaporta, S. L. (1995) Transposon-mediated chromosomal rearrangements and gene duplications in the formation of the maize R-r complex. The EMBO Journal 14: 2350-2363.
Weiher, H., Konig, M., Gruss, P. (1983) Multiple point mutations affecting the Simian virus 40 enhancer. Science 219: 626-631.
Christensen, et al. (1992). Maize polyubiquitin genes: structure, thermal perturbation of expression and transcript splicing, and promoter activity following transfer to protoplasts by electroporation. Plant Mol. Biol. 18:675-689.
Odell, et al. (1985). Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promoter. Nature 313: 810-812.
Depicker, et al. (1982). Nopaline synthase: transcript mapping and DNA sequence. J. Mol. Appl. Genet. 1:561-573.

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