P-anisoyl streptokinase/plasminogen complex

Drug – bio-affecting and body treating compositions – Enzyme or coenzyme containing – Stabilized enzymes or enzymes complexed with nonenzyme

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424 9464, 435174, 435188, 435216, 514802, A61K 3747, A61K 37547, C12N 1100, C12N 970

Patent

active

048084050

ABSTRACT:
A binary complex between streptokinase and plasminogen is prepared in which the catalytic site essential for fibrinolytic activity is blocked by a group which is removable by hydrolysis such that the pseudo-first order rate constant for hydrolysis of the complex is in the range 10.sup.-6 sec.sup.-1 to 10.sup.-3 sec.sup.-1 in isotonic aqueous media at pH 7.4 at 37.degree. C. The complex is preferably a p-anisoyl streptokinase/plasminogen complex without internal peptide bond cleavage. The complex is useful in the treatment of venous thrombosis.

REFERENCES:
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patent: 4285932 (1981-08-01), Smith
McClintock et al., Biochemical and Biophysical Research Communications, vol. 43, No. 3, 1971, pp. 694-702.
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Chase, Jr. et al., Biochem., 8, 2212-2224 (1969).
McClintock et al., Biochem., 13, 5334-5344 (1974).
Markus et al., J. Biol. Chem., 251, 6495 (1976).
Brockaway et al., Biochem., 13, 2063-2069 (1974).
Abstract P5-076 0929, Thrombosis & Haemostasis, (Stuttgart), 42, 390 (1979).

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