Oxygenase enzymes and screening method

Details Oxygenase enzymes and screening method Oxygenase enzymes and screening method

2005-06-07

2005-06-07

Rao, Manjunath (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

C435S004000, C435S006120, C435S025000, C435S069100, C435S183000, C435S252300, C435S320100, C536S023200, C536S023400, C536S023700

Reexamination Certificate

active

06902918

ABSTRACT:
A method for detecting the presence of an oxygenated compound which is produced when a substrate is reacted with an oxygenase for the substrate. The method involves reacting a coupling enzyme with the oxygenated compound to form a polymeric oxygenated compound which is fluorescent or luminescent. Measurement of the fluorescence or luminescence of the polymeric oxygenated compound provides indirect detection of the oxygenated compound produced by reaction of the oxygenase with the substrate. The method is carried out in a whole cell environment wherein the cell is transformed to express both the oxygen a set being screened and the coupling enzyme. The method can be used to measure the activity of monooxygenases and dioxygenases on aromatic substrates. The method is amenable to large scale screening of enzyme mutants to isolate those with maximum oxygenase activity.

REFERENCES:
patent: 5605793 (1997-02-01), Stemmer
patent: 5741691 (1998-04-01), Arnold et al.
patent: 5811238 (1998-09-01), Stemmer et al.
patent: 5830721 (1998-11-01), Stemmer et al.
patent: 5837458 (1998-11-01), Minshull et al.
patent: 5965408 (1999-10-01), Short
patent: 0 505 198 (1992-09-01), None
patent: WO 95/22625 (1995-08-01), None
patent: WO 97/16553 (1997-05-01), None
patent: WO 97/20078 (1997-06-01), None
patent: WO 97/35957 (1997-10-01), None
patent: WO 97/35966 (1997-10-01), None
patent: WO 98/27230 (1998-06-01), None
patent: WO 98/31837 (1998-07-01), None
patent: WO 98/41653 (1998-09-01), None
patent: WO 98/42832 (1998-10-01), None
patent: WO 00/00632 (2000-01-01), None
patent: WO 00/04190 (2000-01-01), None
patent: WO 00/06718 (2000-02-01), None
patent: WO 00/09679 (2000-02-01), None
patent: WO 00/18906 (2000-04-01), None
Manchester et al. Biochimie, 1996, vol. 78:714-722.
Manchester et al. Protein Engineering, 1995, vol. 8(8):801-807.
Chang et al., Nature Biotechnol 1999;17:793-797.
Christians et al., Nature Biotechnol 1999;17:259-264.
Crameri et al., Nature Med 1996;2(1):100-106.
Crameri et al., Nature Biotechnol 1997;15:436-438.
Crameri et al., Nature 1998;391:288-291.
De Haan et al., Biochim Biophys Acta. 1969;180:417-9.
Lipscomb et al., J. Biol. Chem. 1976;251:1116.
Minshull and Stemmer, Curr Opin Chem Biol 1999;2:284-290.
Ness et al., Nature Biotechnol, 1999;17:893-896.
Patten et al., Curr Opin Biotechnol 1997;8:724-733.
Riggs, in Ausubel, F. M., et al. (eds), Current Protocols in Molecular Biology, Greene Associates/Wiley Interscience, New York, 1992.
Stemmer et al., Biotechniques 1992;14:256 et seq.
Stemmer et al., Nature 1994;370:389-391.
Zhang et al., Proc Natl Acad Sci USA 1997;94:4504-4509.
Zylstra and Gibson, J. Bacteriol. 1989;264:14940-14946.
Joo et al., “Laboratory evolution of peroxide-mediated cytochrome P450 hydroxylation”,Nature,vol. 399, No. 6737, Jun. 17, 1999, p. 670-673.
Kuchner et al., “Directed evolution of enzyme catalysts”,Trends in Biotechnology,vol. 15, No. 12, Dec. 1977, p. 523-530.
Oman et al., “Microperoxidase/H-20-2-catalyzed aromatic hydroxylation proceeds by a cytochrome-P-450-type oxygen-transfer reaction mechanism”,European Journal of Biochemistry,vol. 240, No. 1, 1996, p. 232-23.
Vidakovic et al., “Understanding the role of the essential Asp251in cytachrome P450cam using site-directed mutagenesis, crystallography, and kinetic solvent isotope effect”,Biochemistry,vol. 37, No. 26.
Faber, K. Biotransformations in Organic Chemistry Springer-Verlad, Berlin, p. 214-217 (1997).
Cook and AtkinsBiochemistry36, 10801 (1997).
Short, J.,Nature Biotechnol.15, 1322 (1997).
Sheldon, R.A. Catalysis: the key to waste minimization.J. Chem. Tech. Biotechnol.68, 381 (1997).
Gonzalez, F.J. and Nebert, D.W., Evolution of the P450-gene superfamily-animal plant warfare, molecular drive and human genetic differences in drug oxidation.Trends Genet.6, 182-186 (1990).(1975).
Guengerich, F.P., in Cytochrome P450: Structure, Mechanism, and Biochemistry (Ortiz de Montellano, P.R., E.d.) pp. 473-536, Plenum Press, New York (1995).
England et al. L.,FEBS Lett.424, 271 (1998).
Lipscomb et al.,J. Biol. Chem.251, 1116 (1976).
Blake II, R. C. and Coon, M.J. Biol. Chem.,255, 4100 (1980).
van Deurzen et al.,Tetrahedron53, 13183 (1997).
Nordblom et al.,Arch. Biochem. Biophys.175, 524 (1976).
Rahimtula and O'Brien,Biochem. Biophys. Res. Commun.60 440 (1974).
Mueller et al., Twenty-five Years of P450camResearch, inCytochrome P450: Structure, Mechanism, and Biochemistry(2nd ed. Montellano, P. R. O. de), Plenum Press, NY, pp83-124(1995).
Cadwell, R. C. and Joyce, G. F., Randomization of Genes by PCR Mutagenesis, in:PCR Methods&Applications,Cold Spring Harbor Laboratory Press, NY, pp28-33 (1992).
PCT Application No. PCT/US98/05956.
D. R. Thatcher, A. Hitchcock, inMechanisms of Protein FoldingR. H. Pain, Ed. (IRL Press, Oxford, 1994) pp. 229-261.
C. B. Anfinsen,Science181, 223-230 (1973).
C. H. Schein,Bio/Technology8, 308-317 (1990).
A. Mitraki, J. King,FEBS Lett.307, 20-25 (1992).
J. X. Zhang, D. P. Goldenberg,Biochemistry32, 14075-14080 (1993).
Wetzel et al.,Bio/Technology9, 731-737 (1991).
Crameri et al., W. P. C. Stemmer,Nature Biotechnol.14, 315-319 (1996).
S. P. Lei, H. C. Lin, S. S. Wang, J. Callaway, G. Wilcox,J. Bacteriol.169, 4379-4383 (1987).
A. T. Smith, et al.,J. Biol. Chem.265, 13335-13343 (1990).
A. M. Egorov, et al.,Ann. N. Y. Acad. Sci. ,35-40 (1991).
S. A. Ortlepp, D. Pollard-Knight, D. J. Chiswell,J. Biotechnol.11, 353-364 (1989).
F. W. Studier, A. H. Rosenberg, J. J. Dunn, J. W. Dubendorff,Meth. Enzymol.185, 60-89 (1990).
S. Shafikhani, R. A. Siegel, E. Ferrari, V. Schellenberger,Biotechniques23, 304-310 (1997).
K. Sirotkin,J. Theor. Biol.123, 261-279 (1986).
J. S. Shindler, R. E. Childs, W. G. Bardsley,Eur. J. Biochem.65, 325-331 (1976).
J. Carbon, L. Clarke, C. Ilgen, B. Ratzkin, inRecombinant Molecules: Impact on Science and SocietyR. F. J. Beers, E. G. Bassett, Eds. (Raven Press, New York, pp. 355-378 (1977)).
M. Ostermeier, K. Desutter, G. Georgiou,J. Biol. Chem.271, 10616-10622 (1996).
R. Parekh, K. Forrester, D. Wittrup,Protein Expres. Purif.6, 537-545 (1995).
R. D. Gietz, R. H. Schiestl, A. Willems, R. A. Woods,Yeast11, 355-360 (1995).
K. G. Welinder,Eur. J. Biochem.96, 483-502 (1979).
D. B. Goodin, M. G. Davidson, J. A. Roe, A. G. Mauk, and M. Smith, Biochemistry 30, 4953-4962 (1991).
M. M. Fitzgerald, M. J. Churchill, D. E. McRee, and D. B. Goodin, Biochemistry, 33, 3807-3818 (1994).
Miura, Y. and Fulco, A.J.Biochim. Biophys. Acta388, 305 (1975).
Borchardt, J.K., Combinatorial Chemistry: Not just for pharmaceuticals,Today's Chem. at Work,Nov. 1998, pp. 36-39.
Setti et al.,Enzyme Microb. Technolo.22 656-661 (1998).
G. A. Olah & T. D. Ernst, Oxyfunctionalization of Hydrocarbons. 14. Electrophilic Hydroxylation of Aromatics with Bis(trimethylsilyl)peroxide/Triflic Acid,J. Org. Chem.54: 1204-1206 (1989).
J.T. Groves & Y.-Z. Han, Models and Mechanisms of Cytochrome P450 Action, in Cytochrome P450, 2nd Edition, Ed. P. R. Ortiz de Montellano, Plenum, NY pp3-48.
A. H. Hoveyda, Catalyst discovery through combinatorial chemistry,Chemistry&Biology5:R187-R191 (1998).
Stuart Borman, Combinatorial Catalysts,Chemical&Engineering News,Nov. 4, 1996 p. 37-39.
Handelsman, J. et al., Molecular biological access to the chemistry of unknown soil microbes: a new frontier for natural products,Chem.&Biol.,5:R245-249 (1998).
Menage, S. et al., O2activation and aromatic hydroxylation performed by diiron complexes,J. Am. Chem. Soc.,120, 133370-13382 (1998).
Lonergan et al.,FEMS153:485-490 (1997).
Ikeda et al.,Macromolecules29:3053-3054 (1996).
White et al.,Ann. Rev. Biochem.49:315-56 (1980).

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