Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Method of co-culturing cells
Reexamination Certificate
2006-08-15
2010-06-08
Crouch, Deborah (Department: 1632)
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Method of co-culturing cells
C435S325000
Reexamination Certificate
active
07732202
ABSTRACT:
Methods of producing human stem cells are disclosed for parthenogenetically activating human oocytes by manipulation of O2tension, including manipulation of Ca2+under high O2tension and contacting oocytes with serine threonine kinase inhibitors under low O2tension, isolating inner cell masses (ICMs) from the activated oocytes, and culturing the cells of the isolated ICMs under high O2tension. Moreover, methods are described for the production of stems cells from activated oocytes in the absence of non-human animal products, including the use of human feeder cells/products for culturing ICM/stem cells. Stem cells produced by the disclosed methods are also described.
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Janus Jeffrey D.
Kuzmichev Leonid N.
Pryzhkova Marina V.
Revazova Elena S.
Crouch Deborah
DLA Piper (LLP) US
International Stem Cell Corporation
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