Oxygen stabilized enzyme electrode

Chemistry: electrical and wave energy – Processes and products – Vacuum arc discharge coating

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204 1T, 204195P, 435817, C12Q 126

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active

043740138

ABSTRACT:
An enzyme electrode based on an oxygen electrode and an oxygen consuming enzyme is described. The oxygen demand of the electrode is satisfied through an electrolytical oxygen production by means of an anode located close to the enzyme and the sensitive surface of the oxygen electrode. The deviation from a reference value, which could be constant or be formed by the signal from an enzymatically passive oxygen electrode, of the oxygen electrode signal, is used to control the electrolysis current in order to keep the oxygen activity in the enzyme electrode constant. The electrolysis current is used as a measure of the concentration of the substrate of the enzyme.

REFERENCES:
patent: 3539455 (1970-11-01), Clark
patent: 3919052 (1975-11-01), Fresnel et al.
patent: 4016044 (1977-04-01), Fresnel et al.
Williams et al., "Electrochemical-Enzymatic Analysis of Blood Glucose and Lactate", Analytical Chemistry, vol. 42, No. 1, Jan. 1970, pp. 118-121.
Romette et al., "Glucose-Oxidase Electrode, Measurements of Glucose in Samples Exhibiting High Variability in Oxygen Content", Clinica Chimica Acta, 95, (1979), pp. 249-253.
Enfors et al., "Enzyme Electrodes for Fermentation Control", Process Biochemistry, vol. 13, No. 2, 1978.
Keyes et al., "Glucose Analysis Utilizing Immobilized Enzymes", Enzyme Microb. Technol., 1979, vol. 1, Apr., pp. 91-94.
Guilbault et al., "An Enzyme Electrode for the Amperometric Determination of Glucose", Analytica Chimica Acta, 64, (1973), pp. 439-455.

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