Chemistry: molecular biology and microbiology – Apparatus – Bioreactor
Patent
1981-05-21
1983-12-06
Warden, Robert J.
Chemistry: molecular biology and microbiology
Apparatus
Bioreactor
25218825, 422211, 422222, 422305, 423657, 435801, C12M 122, C09K 300, C01B 308, C01B 1516
Patent
active
044194510
ABSTRACT:
A self-contained system is provided for producing an oxygen-free environment for anaerobiosis and culturing wherein the system possesses the ability to provide and maintain the oxygen-free environment, even after the opening and closing of the sealed container therefore several times. The system includes a metallic couple, preferably copper-magnesium, which reacts with water to induce and maintain the oxygen-free environment. The degree of addition of water to the system controls the rate of reduction of the environment. The system possesses a dual oxygen scavenging function by the controlled addition of water. That is, if a small amount of moisture is added to the system couple of the invention, hydrogen is liberated, which causes a drying in the system simultaneously with a reaction of a portion of the couple to gradually scavenge oxygen from the environment, simultaneously with the liberation of hydrogen. If desired, in such an arrangement, the subsequent addition of water generates hydrogen again until all the magnesium is used up, in order to provide a subsequent oxygen scavenging property. In addition, the system includes a sealable petri dish so conformed to include means for maintaining the system of the invention in combination with an indicator for anaerobiosis, a catalyst for the system, a carbon dioxide generator, and space for the culture media.
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Garner Richard L.
Winans, Jr. Luther
Becton Dickinson and Company
Warden Robert J.
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