Oxygen-dissolving process and an apparatus for practicing the pr

Details Oxygen-dissolving process and an apparatus for practicing the pr Oxygen-dissolving process and an apparatus for practicing the pr

1991-09-05

1993-11-23

Sever, Frank

Liquid purification or separation

Processes

Liquid/liquid solvent or colloidal extraction or diffusing...

95 54, B01D 6138

Patent

active

052641317

ABSTRACT:
The present invention provides a process for dissolving oxygen comprising forming a liquid membrane of an oxygen-permeable liquid on a support layer made of a hydrophobic porous material, bringing one side of the liquid membrane into contact with an oxygen-containing gas and the other side with an aqueous liquor, and thereby dissolving oxygen in the aqueous liquor; a process for culturing cells in an oxygen-containing aqueous liquor obtained by the oxygen-dissolving process; and apparatus for practicing these processes. According to the present invention, oxygen can be dissolved in an aqueous liquor efficiently and economically, and aerobic cell-culturing can be stably effected even at a high cell concentration.

REFERENCES:
patent: 3958014 (1976-05-01), Watanabe et al.
patent: 4734112 (1988-03-01), Okita et al.
patent: 4761164 (1988-08-01), Pez et al.
patent: 4853097 (1989-08-01), Marchionni et al.
Develop. biol. Standard., "Optimization of a Membrane Oxygenation System for a Cell Culture in Stirred Tank Reactors", vol. 66, pp. 263-268.
Develop. biol. Standard., "Bubble Free Cell Culture Aeration with Porous Moving Membranes", vol. 66, pp. 227-240.

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