Oxidizing reagent for use in oligonucleotide synthesis

Details Oxidizing reagent for use in oligonucleotide synthesis Oxidizing reagent for use in oligonucleotide synthesis

1995-09-11

1998-07-21

Wilson, James O.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 2531, 423462, 423466, 423486, 4234991, 423500, 423502, C07H 100

Patent

active

057836841

ABSTRACT:
Oxidizing compositions particularly for use in automated oligonucleotide synthesis containing a mixture of KI and I.sub.2 in solution, in equilibrium with KI.sub.3. One preferred composition contains 1.75% KI.sub.3 (providing 0.69% KI and 1.06% I.sub.2) in tetrahydrofuran/pyridine/water (93/5/2, v/v). These formulations enable synthesis of oligonucleotides of significantly higher quality than the currently employed formulation comprising 3% I.sub.2 in tetrahydrofuran/pyridine/water (74/21/2, v/v).

REFERENCES:
Gait, M. J. "Oligonucleotide Synthesis a Practical Approach", IRL Press, p. 50, 1985.
Matteucci, M.D. & Caruthers, M.H., "The Synthesis of Oligodeoxpyrimidines on a Polymer Support", Tetrahedron Letters, vol.. 21, pp. 719-722.
Ogilvie, K. K. & Nemer, M.J., "The Synthesis of Phosphite Analogues of Ribonucleotides"; Tetrahedron Letters, vol. 21, pp.4145-4148.
Ogilvie, K. K. & Nemer, Mona J., "Silica Gel As Solid Support in the Synthesis of Oligoribonucleotides", Tetrahedron Letters, vol. 21, pp. 4159-4161.

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