Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Patent
1995-01-24
1997-10-21
Naff, David M.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
435 41, 435 911, 435101, 435180, 435181, 43524023, 43524024, 436531, 436532, 530412, 530415, 530417, 530815, 530816, 530402, 525383, C12P 2106, C12N 1106, G01N 33549, C07K 1706
Patent
active
056795394
ABSTRACT:
Polyolefin particles are chemically modified by oxidation to provide a large surface area and high loading. The particles result in low back pressure in column systems, and are economical to manufacture. The particles are useful as supports in a wide range of applications including general organic as well as biopolymer synthesis, library methods, purification processes and enzyme mediated processes. In a preferred embodiment, polyethylene or polypropylene particles are oxidized in a solution containing trifluoroacetic acid or trifluoromethane sulfonic acid, chromium trioxide and sulfuric acid to provide the particles with a chemically reactive irregular surface and open channels that extend below the surface and up to essentially the length of the radius of the particles resulting in increased surface area and decreased density. The particles have pendant functional groups produced by the oxidation and/or by subsequent chemical reaction. The pendant functional groups may be attached to spacer arms that are attached to the surface of the particles. Biological material such as proteins, enzymes and whole cells may be immobilized on the particles.
REFERENCES:
patent: 4177253 (1979-12-01), Davies et al.
patent: 4256840 (1981-03-01), Meitzner et al.
patent: 4351909 (1982-09-01), Stevens et al.
patent: 4501826 (1985-02-01), Meitzner et al.
patent: 5174996 (1992-12-01), Frankfurt et al.
patent: 5321094 (1994-06-01), McGee
patent: 5576220 (1996-11-01), Hudson et al.
patent: 5585275 (1996-12-01), Hudson et al.
K.K. Unger and R. Janzen, J. Chromatography, 373, 227-264 (1986).
"Solid Phase Biochemistry, Analytical and Synthetic Aspects", Ed W.H. Scouten, John Wiley, NY, 1983.
Oligonucleotide Synthesis, A Practical Approach, Ed. by M.J. Gait; IRL Press, Oxford, 1984, pp. 35-51 & 81.
G. Jung, A.G. Beck-Singer, Angew. Chem. Int. Ed. Eng. 31, 367 (1992).
G. Barany and R.B. Merrifield in The Peptides, Analysis, Synthesis, Biology, vol. 2A; Ed. E. Gross and J. Meienhofer, Academic Press, NY, 1980 pp. 3-35.
J.R. Hollahan, B.B. Stafford, R.D. Falb and S.T. Payne, J. App. Polymer Sci 13, 807 (1969).
S.R. Holmes-Farley, Robert H. Reamey, Thomas J. McCarthy, John Deutch, and G.M. Whitesides, Langmuir 1, 725-740 (1985).
S.S. Bentjen, D.A. Nelson, B.J. Tarasevich and P.C. Rieke, J. App. Polymer Sci. 44, 965 (1992).
N.R. Shenoy, J.M. Bailey and J.E. Shively, Protein Science 1, 58 (1992).
H.M. Geysen, R.H. Meleon and S.J. Barteling, Proc. Natl. Acad. Sci. USA 81, 3998 (1984).
J. Vagner, V. Krchnak, N.P. Sepetov, P. Strop, K.S. Lam, G. Barany and M. Lebl in "Innovations and Perspectives in Solid Phase Synthesis; Proceedings of the IIIrd International Symposium", R. Epton, Ed., Mayflower, Birmingham, p. 347 (1994).
K.S. Lam, S.E. Salmon, E.M. Hersh, V.J. Hruby, W.M. Kasmierski and R.J. Knapp, Nature 54, 82 (1991).
S. Birnbaum, P-O Larsson and K. Mosbach in, "Solid Phase Biochemistry, Analytical and Synthetic Aspects", Ed W.H. Scouten, John Wiley, NY, 1983, chapter 15.
Cook Ronald M.
Hudson Derek
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