Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1997-10-31
1999-03-09
Hendricks, Keith D.
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
435195, 435198, C12N 1509, C12N 1555, C12N 920
Patent
active
058802720
ABSTRACT:
An oxidized phospholipid degrading enzyme is provided, which plays an important role in the oxygen stress preventive mechanism in organisms. The enzyme hydrolyzes a 1-acyl-2-.omega.-carboxyfatty acid acyl-3-phosphatidylcholine as a substrate at the 2-ester bond thereof to form 1-acyl-2-lyso-3-phosphatidylcholine. The activity of the enzyme is slightly enhanced by 4 mM calcium chloride. The molecular mass of the enzyme is 95.+-.5 kDa (by gel filtration). The enzyme is composed of three subunits whose molecular masses have been found to be 29 kDa, 30 kDa and 45 kDa, respectively, by SDS-polyacrylamide electrophoresis. A gene coding the enzyme is also provided. This gene is important for the synthesis of the enzyme by genetic engineering.
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Adachi Hideki
Arai Hiroyuki
Inoue Keizo
Tsujimoto Masafumi
Hendricks Keith D.
Mayhew Bradley S.
Suntory Limited
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