Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1995-06-06
1998-10-20
Prouty, Rebecca E.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435201, 435203, 435204, 4351723, 435 712, 4352523, 43525231, 4353201, 536 232, 536 237, C12N 1556, C12N 928, C12N 121, C12N 1563
Patent
active
058245324
ABSTRACT:
Novel alpha-amylase mutants derived from the DNA sequences of naturally occurring or recombinant alpha-amylases are disclosed. The mutant alpha-amylases, in general, are obtained by in vitro modifications of a precursor DNA sequence encoding the naturally occurring or recombinant alpha-amylase to generate the substitution (replacement) or deletion of one or more oxidizable amino acid residues in the amino acid sequence of a precursor alpha-amylase. Such mutant alpha-amylases have altered oxidative stability and/or altered pH performance profiles and/or altered thermal stability as compared to the precursor. Also disclosed are detergent and starch liquefaction compositions comprising the mutant amylases, as well as methods of using the mutant amylases.
REFERENCES:
patent: 4261868 (1981-04-01), Hora et al.
patent: 4284722 (1981-08-01), Tamuri et al.
patent: 4493893 (1985-01-01), Mielenz et al.
patent: 4620936 (1986-11-01), Kielman et al.
patent: 4634551 (1987-01-01), Burns et al.
patent: 4732973 (1988-03-01), Barr et al.
patent: 4752585 (1988-06-01), Koths et al.
patent: 4760025 (1988-07-01), Estell et al.
patent: 4863626 (1989-09-01), Coyne et al.
patent: 5118623 (1992-06-01), Boguslawski et al.
patent: 5346823 (1994-09-01), Estell et al.
Y. Matsuura et al., "Structure and Possible Catalytic Residues of Taka-Amylase A", J. Biochemistry 95: 697-702, (1984).
G. Gray et al., "Structural Gene Encoding the Thermophillic .alpha.-Amylases of Bacillus stearothermophilus and Bacillus licheniformis", J. Bact. 166(2) 635-643, (May 1986).
L. Holn et al., "Random Mutagenesis used to Probe the Structure and Function ofBacillus stearothermophilus Alpha Amylase", Prot. Engineering 3(3) 181-191 (1990).
A. Rudolphus et al., "Oxidation-Resistant Variants of Recombinant Antileucoprotease Are Better Inhibitors of Human Neutrophil Elastase Induced Emphysema in Hamsters Than Natural Recombinant Antileucoprotease", Clinical Science 81(6): 777-784, Dec. 1991.
Bealinkelly et al, "Studies on the thermostability of the alpha-amylase of bacillus-caldovelox" Appl. Microbiol. and Biotech 36(3):332-336 (Dec. 1991).
Brosnan, et al., "Investigation of the mechanism of irreversible thermoinactivation of bacillus-stearothermophilus alpha-amylase" Eur. J. of Biochem. 203(1-2)225-231 (Jan. 1992).
Declerck, et al., "Use of Amber Suppressors to Investigate the Thermostability of Bacillus licheniformis .alpha.-Amylase" J. Biol. Chem. 265(26):15481-15488 (1990).
Estell, et al., "Engineering an enzyme by Site-directed Mutagenesis to Be Resistant to Chemical Oxidatin" J. of Biol. Chem. 260(11)6518-6521 (Jun. 1985).
Janecek, et al., ".alpha.-Amylases and approaches leading to their enhanced stability" FEBS 11085 304(1,1-3):1-3 (Jun. 1992).
Jorgenesen, "Cloning of a chromosomal .alpha.-amylase gene from Bacillus stearothermophilus" FEMS Microbiology Letters 77:271-276 (1991).
Joyet, et al., "Hyperthermostable variants of a highly thermostable alpha-amylase" Biotechnology 10:1579-1583 (Dec. 1992).
Manning, et al., "Thermostable .alpha.-Amyulase of Bacillus stearothermophilus" J. of Biol. Chem. 236(11):2952-2965 (Nov. 1961).
Matsui et al., "A mutant .alpha.-amylase with enhanced activity specific for short substrates" FEBS 11596 310(3):216-218 (Oct. 1992).
Matsui et al., "An increase in the transglycosylation activity of Saccharomycopsis .alpha.-amylase altered by site-directed mutagenesis" Biochemica et Biophysica Acta 1077:416-419 (1991).
Nakajima, et al., "Nucleotide Sequence of the Bacillus stearothermophilus .alpha.-Amylase Gene" J. Bacteriology 163(1):401-406 (Jul. 1985).
Ogasahara, et al., "Studies on Thermophilic .alpha.-Amylase from Bacillus stearothermophilus" J. Biochem. 67(1):65-89 (1970).
Ottesen et al., "The Subtilisins" Methods in Enzymology 19:199-215.
Sogaard et al., "Site-directed Mutagenesis of Histidine 93, Aspartic Acid 180, Glutamic Acid 205, Histidine 290, and Aspartic Acid 291 at the Active Site and Tryphtophan 279 at the Raw Starch Binding site in Barley .alpha.-Amylase 1" J. Biol. chem. 268(32) 22480-22484 (Oct. 1993).
Suzuki, et al., "Amino Acid Residues Stabilizing a Bacillus .alpha.-Amylase agaimst Irreversible Thermoinactivation" J. Biol. Chem. 264(32):18933-18938 (Nov. 1989).
Svensson, et al., "Mutational analysis of glucosylase function" J. Biotech. 29:1-37 (1993).
Takase et al., "Site-directed mutagensis of active site residues in Bacillus subtilis a-amylase" Biochimica et Biophysica Acta 1120-281-288 (1992).
Tomazic et al., "Mechansims of Irreversible thermal Inactivation of Bacillus .alpha.-Amylases" J. of Biol. Chem. 262(7):3086-3091 (Mar. 1988).
Vihinen et al. "Site-Directed Mutagenesis of a Thermostable .alpha.-Amylases from Bacillus stearothermophilus: Punitive Role of Three Conserved Residues" J. Biochem 107:267-272 (1990).
Barnett Christopher C.
Mitchinson Colin
Power Scott D.
Requadt Carol A.
Genencor International Inc.
Prouty Rebecca E.
Stone Christopher L.
LandOfFree
Oxidativley stable alpha-amylase does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Oxidativley stable alpha-amylase, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Oxidativley stable alpha-amylase will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-243404