Oxidative method for attachment of glycoproteins or glycopeptide

Coating processes – Medical or dental purpose product; parts; subcombinations;... – Analysis – diagnosis – measuring – or testing product

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4241301, 424 943, 427 212, 427 224, 427 225, 427 23, 427 231, 427338, 435174, 435176, 435181, 435188, 523112, 523113, B05D 310, A61L 2700, A61L 3300

Patent

active

058915060

ABSTRACT:
A method for making a medical device having at least one glycoprotein and/or glycopeptide immobilized on a substrate surface is provided. The method may include oxidizing 1,2 dihydroxy moieties with a periodate to form an aldehyde-functional material; combining the aldehyde-functional material with an amino-functional material to bond the two materials together through an imine moiety; and reacting the imine moiety with a reducing agent to form a secondary amine. Another method of the present invention may be employed to crosslink glycoproteins and/or glycopeptides immobilized on medical device surfaces. Additionally, one method of the present invention may be employed to crosslink glycoproteins and/or glycopeptides, thereby forming a crosslinked biomaterial or a crosslinked medical device coating.

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