Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Chemical modification or the reaction product thereof – e.g.,...
Patent
1996-04-25
1998-10-13
Naff, David M.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Chemical modification or the reaction product thereof, e.g.,...
424422, 424 941, 435174, 435176, 435177, 435181, 436518, 436524, 436528, 436532, 530810, 530811, 530812, 530816, C07K 1700, A61F 200, C12N 1100, G01N 33543
Patent
active
058213430
ABSTRACT:
A method is provided for immobilizing a biomolecule coating on the surface of a medical device to obtain improved biocompatibility characteristics for contacting with tissue or body fluids such as blood. The method includes oxidizing a 2-aminoalcohol moiety of a material disposed on the surface with a periodate to form an aldehyde moiety, combining the aldehyde moiety with an amine moiety of a material to bond the aldehyde moiety to the amine moiety through an imine moiety and reacting the imine moiety with a reducing agent to form the coating immobilized on the surface by an amine linkage. Alternatively, the method includes providing on the surface a coating containing the materials having the 2-aminoalcohol moiety and the amine moiety, applying a periodate to oxidize the 2-aminoalcohol moiety to an aldehyde moiety, allowing the aldehyde moiety to react with the amine moiety through an imine moiety and reducing the imine moiety to form an amine linkage crosslinking the 2-aminoalcohol moiety to the amine moiety. The material containing the 2-aminoalcohol moiety or the amine moiety can be a biomolecule or a substrate surface of a medical device. Biomolecules such as proteins or peptides containing a 2-aminoalcohol moiety can be attached to an aminated surface or an aminated biomolecule can be attached to surface having a 2-aminoalcohol moiety.
REFERENCES:
patent: 3826678 (1974-07-01), Hoffman et al.
patent: 4521564 (1985-06-01), Solomon et al.
patent: 4600652 (1986-07-01), Solomon et al.
patent: 4613665 (1986-09-01), Larm
patent: 4642242 (1987-02-01), Solomon et al.
patent: 4720512 (1988-01-01), Hu et al.
patent: 4786556 (1988-11-01), Hu et al.
patent: 5032666 (1991-07-01), Hu et al.
patent: 5053048 (1991-10-01), Pinchuk
patent: 5077372 (1991-12-01), Hu et al.
patent: 5344455 (1994-09-01), Keogh et al.
patent: 5362852 (1994-11-01), Geoghegan
patent: 5545213 (1996-08-01), Keogh et al.
R.G. Dickinson et al., "A New Sensitive and Specific Test for the Detection of Aldehydes: Formation of 1719-1720 (1970).
K.F. Geoghegan et al., "Site-Directed Conjugation of Nonpeptide Groups to Peptides and Proteins via Periodate Oxidation of a 2-Amino Alcohol. Application to Modification at N-Terminal Serine", Bioconjugate Chem., 3, 138-146 (1992).
A.S. Hoffman et al., Covalent Binding of Biomolecules to Radiation-Grafted Hydrogels on Inert Polymer Surfaces, Trans. Am. Soc. Artif. Intern. Organs, 18, 10-18 (1972).
S. Holmes et al., Amination of Ultra-high Strength Polyethylene using Ammonia Plasma, Composites Science and Technology, 38, 1-21 (1990).
Y. Ito et al., Materials for Enhancing Cell Adhesion by Immobilization of Cell-Adhesive Peptide, J. Biomed. Mat. Res., 25, 1325-1337 (1991).
P.H. O'Farrell, High Resolution Two-Dimensional Electrophoresis of Proteins, J. Biol. Chem., 250, 4007-4021, (1975).
Naff David M.
Patton Harold R.
Woods Thomas F.
LandOfFree
Oxidative method for attachment of biomolecules to surfaces of m does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Oxidative method for attachment of biomolecules to surfaces of m, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Oxidative method for attachment of biomolecules to surfaces of m will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-314113