Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Chemical modification or the reaction product thereof – e.g.,...
Patent
1989-07-10
1991-10-29
Nutter, Nathan M.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Chemical modification or the reaction product thereof, e.g.,...
530403, 530404, 530405, 530406, 530408, 530409, 530410, C07K 300, C07K 1300, C07K 1500, C07K 1700
Patent
active
050617907
ABSTRACT:
A method for conferring or increasing the antigenicity of a disulfide-crosslinked protein by treating the protein with an oxidizing agent, such as periodate, having an oxidation potential sufficient to cleave disulfide linkages. Excess oxidizing agent is then inactivated by addition of a reducing agent. The resulting protein exhibits an increase in its ability to be bound by select antibodies, particularly monoclonal antibodies directed to linear peptide epitopes in the protein.
REFERENCES:
patent: 4033819 (1977-07-01), Helting
patent: 4658022 (1987-04-01), Knowles et al.
patent: 4683136 (1987-07-01), Milich et al.
patent: 4713366 (1987-12-01), Stevens
Elting James J.
Kolbl Heinz
Klawitter A. L.
Molecular Diagnostics, Inc.
Nutter Nathan M.
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