Oxidative creatinine assay

Details Oxidative creatinine assay Oxidative creatinine assay

1993-10-25

1994-12-20

Redding, David A.

Chemistry: analytical and immunological testing

Heterocyclic carbon compound

Hetero-n

436810, 436904, 436164, 435 4, G01N 3370

Patent

active

053745612

ABSTRACT:
The present invention is a method for the detection of creatinine in an aqueous solution, particularly urine, which involves contacting the solution with a soluble cupric salt, a hydroperoxide and an oxidizable indicator which provides a detectable response in the presence of oxygen free radicals and a pseudoperoxidase. The invention is predicated on the discovery that cupric ions form a complex with creatinine which complex exhibits peroxidase like activity.

REFERENCES:
patent: 3710510 (1973-01-01), Tully et al.
patent: 4004368 (1977-01-01), Tully
patent: 5173431 (1992-12-01), Pugia et al.
Hausenbuiller, R. 1978. Soil Science, pp. 73-75; 481-484. William C. Brown, Co.; Dubuque, Iowa.
Black, C. 1968, Soil-Plant Relationships, pp. 92-94. John Wiley & Sons, Inc.; New York.

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