Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1994-05-19
1995-09-26
Redding, David A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
435 25, 435 4, 435 20, 435 19, 435 11, 435 26, 435 14, 435 10, 435 12, 435810, C12Q 128, C12Q 126, C12Q 158, C12Q 144
Patent
active
054533600
ABSTRACT:
A dye couple, comprising 3-methyl-2-benzothiazolinone hydrazone (MBTH) and 8-anilino-l-naphthalenesulfonate (ANS), is used as an indicator in a reaction cascade producing a strong oxidizing agent, such as hydrogen peroxide or other peroxides or perborates. The strong oxidizing agent reacts with the dye couple to produce a blue dye stuff. The MBTH-ANS dye couple exhibits strong and flat spectral absorption at the region of about 600 to 650 nm. This region is free of blood color interference, which enables one to measure glucose and other analytes that react with an oxidase enzyme to produce the strong oxidizing agent, through the use of LED optics, accurately without much optic calibration. Further, the MBTH and ANS are very soluble in aqueous solution, yet become insoluble upon oxidative coupling. The poor solubility minimizes dye fading, thus providing a stable endpoint.
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Leary Louise N.
Lifescan Inc.
Redding David A.
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