Oxidation-resistant muteins of Il-2 and other protein

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Lymphokines – e.g. – interferons – interlukins – etc.

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530820, 530825, 435 6952, 435 6951, 435 695, 424 852, 424 851, 424 854, 424 855, 424 856, 424 857, C07K 1300, A61K 3700

Patent

active

051169436

ABSTRACT:
A biologically active reference therapeutic protein is protected against oxidation by a method involving substituting a conservative amino acid for each methionyl residue susceptible to chloramine T or peroxide oxidation, wherein additional, non-susceptible methionyl residues are not so substituted. The oxidation-resistant mutein so produced is preferably a human mutein of interleukin-2 or interferon-.beta., and the conservative amino acid is most preferably alanine.

REFERENCES:
patent: 4738927 (1988-04-01), Taniguchi et al.
patent: 4760025 (1988-07-01), Estell et al.
patent: 4761375 (1988-08-01), Clark
Zoaller et al., NA, 10, 1982, pp. 64787-6500.
Wells et al., Gene 34, 1985, pp. 315-323.
Bauer et al., Gene 37, 1985, pp. 73-81.
Lee et al., Bochem Biophys Res. Comm 1988, pp. 807-813, vol. 156(2).
Leatherbarrow et al., Protein Engineering, vol. 1, 1986, pp. 7-16.

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