Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1993-10-29
1995-11-07
Saunders, David
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 18, 435 691, 4351721, 435188, 435207, 435963, C12N 938, C12N 1556, G01N 33535
Patent
active
054647479
ABSTRACT:
A mutein of an enzyme acceptor polypeptide fragment of .beta.-galactosidase which is resistant to oxidation is provided. The enzyme acceptor fragment has an amino acid other than cysteine located at position 602 of the natural sequence. An especially preferred substitution is serine. Also provided are a method for producing the novel mutein, a reagent composition comprising the novel mutein, and an immunoassay method for determining an analyte in which the novel mutein recombines with an enzyme donor polypeptide fragment to form enzymatically active .beta.-galactosidase.
REFERENCES:
patent: 5120653 (1992-06-01), Henderson
Boguslawski Sophie J.
Bryant Christopher P.
Clark Scott
Eisenbeis Scott J.
Krevolin Mark
Amick Marilyn L.
Boehringer Mannheim Corporation
Kenemore Max J.
Saunders David
Young D. Michael
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