Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues
Reexamination Certificate
2006-03-21
2006-03-21
Kerr, Kathleen M. (Department: 1656)
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
C530S300000, C530S350000, C435S007100, C435S069100, C435S325000, C435S252300, C435S320100, C514S002600, C514S012200, C514S021800
Reexamination Certificate
active
07015310
ABSTRACT:
The disclosure provides proteins that can be used to determine the redox status of an environment (such as the environment within a cell or subcellular compartment). These proteins are green fluorescent protein (GFP) variants (also referred to as redox sensitive GFP (rosGFP) mutants), which have been engineered to have two cysteine amino acids near the chromophore and within disulfide bonding distance of each other. Also provided are nucleic acid molecules that encode rosGFPs, vectors containing such encoding molecules, and cells transformed therewith. The disclosure further provides methods of using the rosGFPs (and encoding molecules) to analyze the redox status of an environment, such as a cell, or a subcellular compartment within a cell. In certain embodiments, both redox status and pH are analyzed concurrently.
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Hanson George T.
Remington S. James
Kerr Kathleen M.
Klarquist & Sparkman, LLP
Robinson Hope
The State of Oregon Acting by and through the State Board of Hig
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