Oxidation indicators comprising 3,3',5,5'-tetraalkylbenzidine co

Details Oxidation indicators comprising 3,3',5,5'-tetraalkylbenzidine co Oxidation indicators comprising 3,3',5,5'-tetraalkylbenzidine co

1981-02-23

1983-05-24

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 10, 435 11, 435 14, 435 25, 435805, 436 95, 436135, 436810, C12Q 128, C12Q 126, C12Q 160, C12Q 162

Patent

active

043851142

ABSTRACT:
The detection and determination of (a) hydrogen peroxide or of a substance which reacts with the formation of hydrogen peroxide or (b) of peroxidase or a peroxidatively active substance, is carried out by contacting an unknown sample with a chromogen and, in case (a), with a peroxidatively active substance or in case (b) with hydrogen peroxide or a substance which reacts with the formation of hydrogen peroxide, and evaluating the coloration produced by the reaction, wherein, as the chromogen, there is used at least one 3,3',5,5'-tetraalkylbenzidine compound of the formula: ##STR1## wherein R.sub.1, R.sub.2, R.sub.3 and R.sub.4, which can be the same or different, are straight-chained or branched alkyl radicals containing up to 6 and preferably up to 4 carbon atoms.

REFERENCES:
patent: 2290436 (1942-07-01), Kamlet
patent: 3413197 (1968-11-01), Fraser
patent: 3418083 (1968-12-01), Rey et al.
patent: 3627697 (1971-12-01), Rey et al.
patent: 3630847 (1968-07-01), Rey et al.
patent: 3770381 (1973-11-01), Schmitt et al.
patent: 3926732 (1975-12-01), Rosen et al.
Holland et al., Tetrahedron, 30, 3299-3302 (1974).

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