Oxidase-producing aspergillus niger

Details Oxidase-producing aspergillus niger Oxidase-producing aspergillus niger

1997-09-17

1999-02-02

Witz, Jean C.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Fungi

435190, 435917, C12N 114

Patent

active

058664068

ABSTRACT:
To provide increased levels of oxidase, mutants of Aspergillus niger that constitutively synthesize glucose oxidase when grown in a medium having less than 0.1M (molar) glucose are obtained. To obtain mutants, a microorganism is cultured with a substrate having a nutrient, an auxiliary carbon source and a pH-sensitive indicator; and cultures in which the pH has changed as indicated by the pH-sensitive indicator are selected for further growth. More specifically, Aspergillus niger that has been subjected to mutagens is cultured with an auxiliary carbon source, methyl red and D-glucose at a low concentration below that which will trigger the production of D-glucose oxidase in the microorganism. Portions of the culture indicated by red are separated and increased. One such mutants has accession number NRRL 18927.

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Markwell et al., "Aspergillus niger Mutants with Increased Glucose Oxidase Production", Appl. Microbiol. Biotech., 1989, 30:166-169.
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