Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues
Patent
1988-06-23
1991-05-14
Doll, John
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
530387, 530397, 530398, 514 21, C07K 1500
Patent
active
050157299
ABSTRACT:
A dimeric protein with inhibin activity is isolated from ram reta testis fluid using reverse-phase high-performance liquid chromatography and gel filtration. The isolated molecule is composed of two chains having apparent molecular weights of about 18,000 and about 16,500 to 14,500 Daltons, as measured by gel electrophoresis, which are bound together by disulfide bonding and the longer of which is likely glycosylated. Microsequencing revealed the NH.sub.2 -terminal portion of the longer chain to be Ser- Thr-Pro-Pro-Leu-Pro-Trp-Pro-Trp-Ser-Pro-Ala-Ala-Leu-Arg-Leu-Gln-Arg-Pro-Pr o- Glu-Glu-Pro-Ala-Ala-His-Ala-Asp-Cys and of the shorther chain to by Gly-Leu-Glu-Cys-Asp-Gly-Lys-Val-Asn-Ile-Cys-Cys-Lys-Lys-Gln-Phe. This dimeric protein specifically inhibits basal secretion of FSH, but not of LH, in a rat anterior pituitary monolayer culture system, exhibiting a half-maximal effective dose of about 0.1 to 0.3 ng/ml. Furthermore, antibodies raised against synthetic replicates of the N-terminal six residues of the longer chain, as well as against the N-terminal 25 residues, are effective to reduce the activity of highly purified intact ovine inhibin and might be used to exert a profertility effect in rams, ewes and other mammals.
REFERENCES:
patent: 4409139 (1983-10-01), Ling et al.
patent: 4737578 (1988-04-01), Evans et al.
patent: 4740587 (1958-04-01), Ling et al.
Baker et al., "Studies on the Purification of Inhibin from Ovine Rete Testis Fluid," Adv. Bioscience, 82, pp. 123-132 (1982).
Baker et al., "Studies on the Purification of Ovine Inhibin," Annals N.Y. Acad. Sci, 383, pp. 329-341 (1982).
Al-Obaidi et al., "Suppression of Ovine Plasma FSH by Bovine Follicular Fluid: etc.," J. Endocr., 111, pp. 1-5 (1986).
D. M. Robertson et al., "Isolation of Inhibin from Bovine Follicular Fluid," B.B.R.C., 126, No. 1, pp. 220-226 (1985).
Chem. Abs., vol. 97, 1982, 66654a.
Chem. Abs., vol. 96, 1982, 211060g.
Chem. Abs., vol. 105, 1986, 165141w.
Bardin C. Wayne
Rivier Jean E. F.
Spiess Joachim
Vale, Jr. Wylie W.
Doll John
The Salk Institute for Biological Studies
Wessendorf T. D.
LandOfFree
Ovine inhibin does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Ovine inhibin, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Ovine inhibin will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1649281