Overexpression, purification and characterization of a...

Details Overexpression, purification and characterization of a... Overexpression, purification and characterization of a...

2008-01-15

2008-01-15

Swope, Sheridan (Department: 1652)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

C435S068100, C435S196000

Reexamination Certificate

active

10545905

ABSTRACT:
Compositions for an alkaline phosphatase and methods for over-expression and purification of thermolabile Antarctic phosphatase (TAP) are provided. Uses for TAP include dephosphorylation of nucleic acids, sugars, peptides and proteins. TAP as described herein has advantages over phosphatases from other sources with respect to thermolability at 65° C. and efficiency of dephosphorylation activity at approximately neutral pH.

REFERENCES:
patent: 5707853 (1998-01-01), Millian
patent: 5773226 (1998-06-01), Millian
patent: 5891699 (1999-04-01), Boulain et al.
patent: 6379940 (2002-04-01), Moffett et al.
patent: 6387634 (2002-05-01), Moffett et al.
patent: 0 352 894 (1990-01-01), None
patent: 0441252 (1997-09-01), None
patent: 0 780 466 (2001-06-01), None
patent: WO 01/81365 (2001-11-01), None
International Search Report Jul. 25, 2005.
Reid et al. The Enzymes, vol. IV ch. 17, p. 373 (1971).
Sambrook et al. Molecular Cloning, A Laboratory Manual Sections 1.53-1.72 (1989).
Piesecki et al. Biotechnol. Bioeng. 42: 178-184 (1993).
Bennett, et al.J. Biol. Chem.,271(29): 17006-17012 (1996).
Chen, et al.Acta Biochimica et Biophysica Sinica,28(5): 523-530 (1996).
Rina, et al.Eur. J. Biochem.,267:1230-1238 (2000).
Zhang, et al.Analytical Biochemistry,292: 307-310 (2001).
Wenner, et al.Analytical Biochemistry,268: 201-212 (1999).

Affiliated with

Also associated with

Rating

Overexpression, purification and characterization of a... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Overexpression, purification and characterization of a..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Overexpression, purification and characterization of a... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3923138