Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1990-11-02
1993-03-23
Wax, Robert A.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435190, 4352523, 43525233, 435248, 935 39, 935 43, 935 61, 935 72, 935 73, C12N 1500, C12N 1553, C12N 1567, C12N 1570
Patent
active
051963175
ABSTRACT:
The invention relates to a process for achieving high, thermally-induced expression rates, which last for a relatively long period of time, of proteins in recombinant cells, preferably in E. coli cells, by optimizing cell density, induction temperature and supplemented amino-acid-containing substrates.
REFERENCES:
patent: 4894334 (1990-01-01), Ben-Bassat et al.
Rinas, U., et al., 1989, Applied Microbiology and Biotechnology 31:163-167.
Okita, B., et al. 1989, Biotechnology and Bioengineering 34:854-862.
Kopetzki, E., et al., 1989, Molecular and General Genetics 216:149-155.
Sugimoto, S., et al., 1987, Journal of Biotechnology 5:237-253.
Osburne, M. S., et al., 1985, Journal of Bacteriology 163(3):1101-1108.
Remaut, E., et al., 1983, Nucleic Acids Research, 11(14):4677-4688.
Brummer Wolfgang
Ebeling Wolfgang
Heinrich Martin
Merck Patent Gesellschaft mit beschrankter Haftung
Moore William
Wax Robert A.
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