Over-expression and purification of a truncated thermostable DNA

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease

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4353201, 4352523, 435325, 435419, 536 232, 536 234, C12N 912, C12N 1554, C12N 1562

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active

058145060

ABSTRACT:
The present invention relates to the cloning and purification of a thermostable DNA polymerase, Bst polymerase I from Bacillus stearothermophilus. More specifically, it provides a novel method for producing a truncated Bst polymerase using recombinant DNA and protein fusion techniques.

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