Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Bacterium or component thereof or substance produced by said...
Patent
1996-08-16
1999-12-21
Housel, James C.
Drug, bio-affecting and body treating compositions
Antigen, epitope, or other immunospecific immunoeffector
Bacterium or component thereof or substance produced by said...
4241841, 4242341, A61K 3900, A61K 3902
Patent
active
060045620
ABSTRACT:
An isolated and purified outer membrane protein B1, and peptides formed therefrom, of Moraxella catarrhalis are described. A method for the isolation and purification of outer membrane protein B1 from a bacterial strain that produces B1 protein, e.g. Moraxella catarrhalis, comprises growing the bacteria in culture in iron-depleted medium to enhance the expression of the B1 protein, harvesting the bacteria from the culture, extracting from the harvested bacteria a preparation substantially comprising an outer membrane protein preparation, contacting the outer membrane preparation with an affinity matrix containing immobilized transferrin wherein B1 protein binds to the transferrin, and eluting the bound B1 protein from the transferrin. Disclosed are the uses of the B1 protein as an immunogen for vaccine formulations, and as antigens in diagnostic immunoassays.
REFERENCES:
Yu et al., "The interaction between human transferrin and transferrin binding protein 2 from Moraxella catarrhalis differs from that of other human pathogens", Microbial Pathogenesis, vol. 15, pp. 433-445, 1993.
Sethi, et al., "Serum Antibodies to Outer Membrane Proteins of Moraxella catarrhalis in Patients with Bronchiectasis: Identification of OMP B1 as an Important Antigen", Infection and Immunity, vol. 63, pp. 1516-1520, 1995.
Campagnari et al., "Growth of Moraxella catarrhalis with Human Transferrin and Lactoferrin: Expression of Iron-Repressible Proteins without Siderophare Production", Infection and Immunity, vol. 62, No. 11, pp. 4909-4914, 1994.
Murphy. Microbiological reviews 60(2): 267-279, 1993 (Jun.).
Campagnari et al. Infection and Immunity 62(11): 4909-4914, 1994.
Sethi et al. Infection and Immunity 63(4): 1516-1520, 1995.
Immunization Practices Advisory Committee, Clin. Phar. 8: 839-850, 1989.
Mackett et al. Human Vaccines and Vaccination, B10 S Scientific Publishers, Ltd., UK. 1995.
Housel James C.
Ryan V.
The Research Foundation of the State University of New York
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