Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1995-06-07
1997-08-12
Zitomer, Stephanie W.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C12Q 168
Patent
active
056564309
ABSTRACT:
The present invention provides several methods employing nucleic acid repair enzymes. The present invention provides a method for detecting point mutations in nucleic acid sequences. The present invention further provides a method for detecting non-mutated or wild-type nucleic acid sequences. The present invention also enhances target polynucleotide detection using an oscillation reaction and tail labeling techniques. The present invention also provides helix destabilizing molecule and similar molecules to enhance the hybridization of the probe to the target polynucleotides. Finally, this invention provides a method for determining the repair index for a mismatched or damaged oligonucleotide probe.
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Youil et al. "Screening for mutations by enzyme mismatch cleavage with T4 endonuclease VII", Pro. Natl. Acad. Sci. USA, vol. 92, pp.87-91 (1995).
Corey et al. "Sequence-Selective Hydrolysis of Duplex DNA by an Oligonucleotide-Directed Nuclease," J. Am. Chem. Soc. 1989, vol. 111, pp. 8523-8525 (1989).
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Chirikjian Jack G.
Collier G. Bruce
Trevigen, Inc.
Whisenant Ethan
Zitomer Stephanie W.
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