Orthoester protecting groups in RNA synthesis

Details Orthoester protecting groups in RNA synthesis Orthoester protecting groups in RNA synthesis

1995-06-09

1999-03-30

Kunz, Gary L.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 253, 536 261, C07H 100, C07H 102, C07H 2102

Patent

active

058891366

ABSTRACT:
Phosphoramidite oligonucleotide synthesis is facilitated by the use of fluoride-labile 5' silyl protecting groups. RNA synthesis is improved by the use of 2' orthoester protecting groups. Reactions are conducted on a solid phase support, and acidic deprotection conditions are avoided, as is the necessity of oxidizing the phosphite linkage between each coupling reaction.

REFERENCES:
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