Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1998-07-17
2000-09-05
Patterson, Jr., Charles L.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
4352523, 536 232, C12N 942, C12N 1556
Patent
active
061141585
ABSTRACT:
A cDNA (1,520 bp), designated celF, consisting of an open reading frame (ORF) encoding a polypeptide (CelF) of 432 amino acids was isolated from a cDNA library of the anaerobic rumen fungus Orpinomyces PC-2 constructed in Escherichia coli. Analysis of the deduced amino acid sequence showed that starting from the N-terminus, CelF consists of a signal peptide, a cellulose binding domain (CBD) followed by an extremely Asn-rich linker region which separate the CBD and the catalytic domains. The latter is located at the C-terminus. The catalytic domain of CelF is highly homologous to CelA and CelC of Orpinomyces PC-2, to CelA of Neocallimastix patriciarum and also to cellobiohydrolase IIs (CBHIIs) from aerobic fungi. However, Like CelA of Neocallimastix patriciarum, CelF does not have the noncatalytic repeated peptide domain (NCRPD) found in CelA and CelC from the same organism. The recombinant protein CelF hydrolyzes cellooligosaccharides in the pattern of CBHII, yielding only cellobiose as product with cellotetraose as the substrate. The genomic celF is interrupted by a 111 bp intron, located within the region coding for the CBD. The intron of the celF has features in common with genes from aerobic filamentous fungi.
REFERENCES:
Beguin, P. (1983) "Detection of Cellulase Activity in Polyacrylamide Gels Using Congo Red-Stained Agar Replicas" Analytical Biochem. 131::333-336.
Black et al. (1994) "Xylanase B from Neocallimastix patriciarum contains a non-catalytic 455-residue linker sequence comprised of 57 repeats of an octapeptide" Biochem. J. 299:381-387.
Borneman et al. (1989) "Fermentation Products and Plant Cell Wall-Degrading Enzymes Produced by Monocentric and Polycentric Anaerobic Ruminal Fungi" Applied and Environ. Microbiol. 55:1066-1073.
Chen et al. (1998) "Two genes of the anaerobic fungus Orpinomyces sp. strain PC-2 encoding cellulases with endoglucanase activities may have arisen by gene duplication" FEMS Microbiol. Letts. 159:63-68.
Chen et al. (1997) "Sequencing of a 1,3-1,4-.beta.D-Glucanase (Lichenase) from the Anaerobic Fungus Orpinomyces Strain PC-2: Properties of the Enzyme Expressed in Escherichia Coli and Evidence that the Gene Has a Bacterial Origin" J. Bacteriol. 179:6028-6034.
Chen et al. (1995) "A cyclophilin from the polycentric anaerobic rumen fungus Orpinomyces sp. strain PC-2 is highly homologeous to vertebrate cyclophilin B" Proc. Natl. Acad. Sci. USA 92:2587-2591.
Choi, S.--K. And Ljungdahl, L.G. (1996) "Structural Role of Calcium for the Organization of the Cellulosome of Clostridium thermocellum" Biochemistry 35:4906-4910.
Dalrymple et al. (1997) "Three Neocallimastix patriciarum esterases associated with the degradation of complex polysaccharides are members of a new family of hydrolases" Microbiology 143:2605-2614.
Denman et al. (1996) "Characterization of a Neocallimastix patriciarum Cellulase CDNA (celA) Homologous to Trichoderma reesei Cellobiohydrolase II" Appl. Environ. Microbiol. 62:1889-1896.
Dijkerman et al. (1996) "Adsorption Characteristics of Cellulolytic Enzymes from the Anaerobic Fungus Piromyces Sp. Strain E2 on Microcrystalline Cellulose" Appl. Environ. Microbiol. 62:20-25.
Durand et al. (1996) "Molecular characterization of xyn3, a member of the endoxylanase multigene family of the rumen anaerobic fungus Neocallimastix frontalis" Curr. Genet. 30:531-540.
Fanutti et al. (1995) "The Conserved Noncatalytic 40-Residue Sequence in Cellulases and Hemicellulases from Anaerobic Fungi Functions as a Protein Docking Domain" J. Biol. Chem. 270:29314-29322.
Filho (1996) "Purification and characterization of a .beta.-glucosidase from solid-state cultures of Humicola grisea var. thermoidea" Can. J. Microbiol. 42:1-5.
GenBank Accession No. U97153, released in Apr. 1998.
GenBank Accession No. U97154, Submitted Apr. 11, 1997.
GenBank Accession No. AF031934, Submitted Oct. 29, 1997.
Gilbert et al. (1992) "Homologous catalytic domains in a rumen fungal xylanase: evidence for gene duplication and prokaryotic origin" Mol. Microbiol. 6:2065-2072.
Gilkes et al. (1991) "Domains in Microbial .beta.-1,4-Glycanases: Sequence Conservatin, Function, and Enzyme Families" Microbiol. Rev. 55:303-315.
Harjunpaa et al. (1996) "Cello-oligosaccharide hydrolysis by cellobiohydrolase II from Trichoderma reesei" Eur. J. Biochem240:584-591.
Henrissat, B. and Bairoch, A. (1993) "New families in the classification of glycosyl hydrolases based on amino acid sequence similarities"Biochem. J. 293:781-788.
Hoffren et al. (1995) "Molecular dynamics simulation of fungal cellulose-binding domains: differences in molecular rigidity but a preserved cellulose binding surface" Protein Eng. 8:443-450.
Koivula et al. (1996) "The active site of Trichoderma reesei cellobiohydrolase II: the role of tyrosine 169" Protein Eng. 9:691-699.
Li et al. (1997) "Monocentric and Polycentric Anaerobic Fungi Produce Structurally Related Cellulases and Xylanases" Appl. Environ. Microbiol. 63:628-635.
Li et al. (1997) "Two Cellulases, CelA and CelC, from the Polycentric Anaerobic Fungus Orpinomyces Strain Pc-2 Contain N-Terminal Docking Domains for a Cellulase-Hemicellulase Complex" Appl. Environ. Microbiol. 63:4721-4728.
Liu et al. (1997) "An endoglucanase from the anaerobic fungus Orpinomyces joyonii: characterization of the gene and its product" Can. J. Microbiol. 43:477-485.
Millward-Sadler et al. (1996) "Evidence that the Piromyces gene family encoding endo-1,4-mannanases arose through gene duplication" FEMS Microbiol. Letts. 141:183-188.
Pages et al. (1997) "Species-Specificity of the Cohesin-Dockerin Interaction Between Clostridium thermocellum and Clostridium cellulolyticum: Prediction of Specificity Determinants of the Do kerin Domain" Proteins 29:517-527.
Rouvinen et al. (1990) "Three-Dimensional Structure of Cellobiohydrolase II from Trichoderma reesei" Science 249:380-386.
Sheppard et al. (1994) "The use of conserved cellulase family-specific sequences to clone cellulase homologue cDNAs from Fusarium oxysporum "Gene 150:163-167.
Teeri et al. (1987) "Homologous domains in Trichoderma reesei cellulolytic enzymes: gene sequence and expression of cellobiohydrolase II" Gene 51:;43-52.
Van Tilbeurgh et al. (1984) "Separation of endo- and exo-type cellulases using a new affinity chromatography method" FEBS Letts. 1699:215-218.
Wilson, C.A., and Wood, T.M. (1992) "The anaerobic fungus Neocallimastix frontalis: isolatiion and properties of a cellulosome-type enzyme fraction with the capacity to solubilize hydrogen-bond-ordered cellulose" Appl. Microbiol. Biotechnol. 37:125-129.
Zhou et al. (1994) "Intronless celB from the anaerobic fungus Neocallimastix patriciarum encodes a modular family A endoglucanase" Biochem. J. 297:359-364.
Chen Huizhong
Li Xin-Liang
Ljungdahl Lars G.
Patterson Jr. Charles L.
University of Georgia Research Foundation Inc.
LandOfFree
Orpinomyces cellulase celf protein and coding sequences does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Orpinomyces cellulase celf protein and coding sequences, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Orpinomyces cellulase celf protein and coding sequences will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2211209