Orientia tsutsugamushi truncated recombinant outer membrane...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C435S007200, C435S007320, C435S007920

Reexamination Certificate

active

07820395

ABSTRACT:
A scrub typhus diagnostic method and vaccine using a composition comprising truncated r47 protein and truncated r56 protein is disclosed. Vaccines composed of r56 protein variants are also disclosed. Methods of reducing HIV viral loads using r47 and r56 proteins and antibodies raised against r47 and r56 are also disclosed.

REFERENCES:
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patent: 6699674 (2004-03-01), Ching et al.
patent: 7306808 (2007-12-01), Ching et al.
patent: 7335477 (2008-02-01), Ching et al.
patent: WO 99/34215 (1999-07-01), None
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Kelly DJ, Chan CT, Paxton H, et al. Comparative evaluation of a commercial enzyme immunoassay for the detection of human antibody to Rickettsia typhi. Clin Diagn Lab Immunol 1995;2:356-60.
Ching, WM., M. Carl, and GA. Dasch. Mapping of monoclonal antibody binding sites on CNBr fragments of the S-Layer protein antigens of Rickettsia typhi and R. Prowazekii. Mol. Immunol. 1992, 29:95-105.
Dasch, GA., JR. Samms, and JC. Williams. Partial purification and characterization of the major species-specific protein antigens of Rickettsia typhi and rickettsia prowazekii identified by rocket immunoelectrophoresis. Infec. Immun. 1981, 31:276-288.
Seong et al., Neutralization Epitopes on the Antigenic Domain II of theOrientia tsutsugamuchi56-kDa Protein Revealed by Monoclonal Antibodies, Vaccine, 2001, vol. 19, pp. 2-9.
Hickman et al. Murine T-cell Response to Native and Recombinant Protein Antigens of Rickettsiatsutsugamushi, Infection and Immunity, May 1993, vol. 61, No. 5; pp. 1974-1681.
Ching et al., Expression and Refolding of Truncated Recombinant Major Outer Membrane Protein Antigen (r56) ofOrientia tsutsugamushiand Its Use in Enzyme-Linked Immunosorbent Assays, Clinical Diagnostic Labortary Immunology, Jul. 1998, vol. 5 No. 4, pp. 519-526.
Ching, et al., Early diagnosis of Scrub Typhus with a Rapid Flow Assay Using Recombinant Major Outer Membrane Protein Antigen (r56) ofOrientia tsutsugamushi, Clinical and Laboratory Immunology, Mar 2001, vol. 8, No. 2, pp. 409-414.

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