Organotypic intestinal culture and methods of use thereof

Details Organotypic intestinal culture and methods of use thereof Organotypic intestinal culture and methods of use thereof

2007-05-15

2007-05-15

Chen, Shin-Lin (Department: 1632)

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Solid support and method of culturing cells on said solid...

C435S371000, C435S373000, C435S384000, C435S395000

Reexamination Certificate

active

10485283

ABSTRACT:
An organotypic culture comprises an artificial stroma overlayed with epithelial cells isolated from a human colon or intestine. The stroma comprises a mixture of collagen and human fibroblasts isolated from a human colon or intestine. The culture contains a factor that binds the IGF-1 receptor, a factor that binds the EGF receptor, and a factor that binds the LIF receptor. These factors may be added exogenously to the culture via medium or may be expressed by various recombinantly engineered cell types in the culture. The organotypic culture can result in growth that is in situ-like or emphasizes other physiological or morphological states, depending on the balance of factors in the growth media. The organotypic culture may be used in methods for screening of therapeutic, carcinogenic, or growth enhancement factors, or for treating intestinal injuries by applying to the site of an injury the intact culture or the components thereof.

REFERENCES:
patent: 3962125 (1976-06-01), Armstrong
patent: 5073492 (1991-12-01), Chen et al.
patent: 5194596 (1993-03-01), Tischer et al.
patent: 5219739 (1993-06-01), Tischer et al.
patent: 5480975 (1996-01-01), Goldberg et al.
patent: 5661132 (1997-08-01), Eriksson et al.
patent: 5731190 (1998-03-01), Wickham et al.
patent: 5792453 (1998-08-01), Hammond et al.
patent: 5869037 (1999-02-01), Crystal et al.
patent: 5932540 (1999-08-01), Hu et al.
patent: 5962427 (1999-10-01), Goldstein et al.
patent: 5965125 (1999-10-01), Mineau-Hanschke
patent: 5980887 (1999-11-01), Isner et al.
patent: 6040157 (2000-03-01), Hu et al.
patent: 6121246 (2000-09-01), Isner
patent: 6187767 (2001-02-01), Araneo et al.
patent: 9851638 (2001-05-01), Herlyn
patent: 6486133 (2002-11-01), Herlyn et al.
patent: 6521225 (2003-02-01), Srivastava
patent: 2004/0031067 (2004-02-01), Herlyn
patent: 476983 (1992-03-01), None
patent: 550296 (1993-07-01), None
patent: 506477 (1999-06-01), None
patent: WO-93/13807 (1993-07-01), None
patent: WO-95/04142 (1995-02-01), None
patent: WO-95/24473 (1995-09-01), None
patent: WO-95/32708 (1995-12-01), None
patent: WO-96/13597 (1996-05-01), None
patent: WO-96/23065 (1996-08-01), None
patent: WO-96/26736 (1996-09-01), None
patent: WO-96/26742 (1996-09-01), None
patent: WO-96/27006 (1996-09-01), None
patent: WO-96/39508 (1996-12-01), None
patent: WO-96/39515 (1996-12-01), None
patent: WO-97/12050 (1997-04-01), None
patent: WO-97/13857 (1997-04-01), None
patent: WO-97/38729 (1997-10-01), None
patent: WO-98/19712 (1998-05-01), None
patent: WO-98/39035 (1998-09-01), None
patent: WO-98/39055 (1998-09-01), None
patent: WO-99/46364 (1999-09-01), None
patent: WO-99/52356 (1999-10-01), None
patent: WO-00/06195 (2000-02-01), None
patent: WO-01/40455 (2001-06-01), None
patent: WO-02/30443 (2002-04-01), None
patent: WO-03/018752 (2003-03-01), None
Day, R., 2006, Current Stem Cell Research & Therapy, vol. 1, No. 1, pp. 113-120.
Baibakov et al., “Organotypic growth and differentiation of human mammary gland in sponge-gel matrix supported histoculture”,In Vitro Cell Dev. Biol. Anim.Aug. 1994 30A(8):490-495.
Bekku et al., “Carbonic anhydrase I and II as a differentiation marker of human and rat colonic enterocytes”,Res. Exp. Med. (Berl)Dec. 1998 198(4):175-185.
Caesar et al., “A comparison of the specificity of phosphatidylcholine synthesis by human fetal lung maintained in either organ or organotypic culture”,J. Biochem.Jul. 15, 1988 253(2):451-457.
Dahlqvist et al., “Development of the intestinal disaccharidase and alkaline phosphatase activities in the human fetus”,Clin. Sci.Jun. 1966 30(3):517-528.
Evans et al., “Primary cultures for studies of cell regulation and physiology in intestinal eipthelium”,Ann. Rev. Physiol.1994 56:399-417.
Fritsch et al., “Cytokines modulate fibroblast phenotype and epithelial-stroma interactions in rat intestine”,GastroenterologyMar. 1997 112(3):826-838.
Halttunen et al., “Fibroblasts and transforming growth factor beta induce organization and differentiation of T84 human epithelial cells”,GastroenterologyNov. 1996 111(5):1252-1262.
Ho S.B., “Cytoskeleton and other differentiation markers in the colon”,J. Cell. Biochem. Suppl.1992 16G:119-128.
Karamuk et al., “Partially degradable film/fabric composites: textile scaffolds for liver cell culture”,Artif. OrgansSep. 1999 23(9):881-884.
Kaye et al., “Colonic pericryptal fibroblast sheath: replication, migration, and cytodifferentiation of a mesenchymal cell system in adult tissue, II. Fine structural aspects of normal rabbit and human colon”,GastroenterologyMay 1968 54(5):852-865.
Kedinger et al., “Intestinal tissue and cell cultures”,Differentiation1987 36(1):71-85.
Kedinger et al., “Intestinal epithelial-mesenchymal cell interactions”,Ann. NY Acad. Sci.Nov. 17, 1998, 859:1-17.
Menard et al., “Human intestinal brush border membrane hydrolases”, inMembrane Physiopathology(G.B. ed.) Kluwer Academic Publishers, Oct. 1994 pp. 319-731.
Moyer M. P., “Tumor cell culture”,Methods Enzymol.1995 524:153-165.
Oda et al., “Organotypic culture of human gallbladder epithelium”,Exp. Mol. Pathol.Aug. 1995 63(1):16-22.
Oda et al., “Reconstituted human oral and esophageal mucosa in culture”,In Vitro Cell. Dev. Biol. Anim.Jan. 1998 34(1):46-52.
Oda et al., “Culture of human main pancreatic duct epithelial cells”,In Vitro Cell. Dev. Biol. Anim.Mar. 1998 34(3):211-216.
Pageot et al., “Human cell models to study small intestinal functions: recapitulation of the crypt-villus axis”,Microsc. Res. Tech.May 15, 2000 49(4):394-406.
Parenteau et al., “Epidermis generated in vitro: practical considerations and applications”,J. Cell. Biochem.Mar. 1991 45(3):245-251.
Pedersen et al., “A simple method to establish short-term cultures of normal human colonic epithelial cells from endoscopic biopsy specimens. Comparison of isolation methods, assessment of viability and metabolic activity”,Scand. J. Gastroenterol.Jul. 2000 35(7):772-780.
Perreault et al., “Use of the dissociating enzyme thermolysin to generate viable human normal intestinal epithelial cell cultures”,Exp. Cell. Res.May 1, 1996 224(2):354-364.
Perreault et al., “Primary cultures of fully differentiated and pure human intestinal epithelial cells”,Exp. Cell. Res.Nov. 25, 1998 245(1):34-42.
Quaroni et al., “Establishment and characterization of intestinal epithelial cell cultures”,Methods Cell. Biol.1980 21B:403-427.
Quaroni et al., “Cell dynamics and differentiation of conditionally immortalized human intestinal epithelial cells”,GastroenterologyOct. 1997 113(4):1198-1213.
Rogler et al. “Establishment of long-term primary cultures of human small and large intestinal epithelial cells”,Lab. Invest.Jul. 1998 78(7):889-890.
Rousset M., “The human colon carcinoma cell lines HT-29 and Caco-2: two in vitro models for the study of intestinal differentiation”,BiochimieSep. 1986 68(9):1035-1040.
Schorkhuber et al., “Survival of normal colonic epithelial cells from both rats and humans is prolonged by coculcute with rat embryo colonic fibroblasts”,Cell. Biol. Toxical.Jun. 1998 14(3):211-223.
Whitehead et al., “A method for the isolation and culture of human colonic crypts in collagen gels”,In Vitro Cell Dev. Biol.Jun. 1987 23(6):436-442.
Whitehead et al., “Clonogenic growth of epithelial cells from normal colonic mucosa from both mice and humans”,GastroenterologyOct. 1999 117(4):858-865.
Lifebvre et al., “Developmental expression and cellular origin of the laminin aphla2, aplha4, and alpha5 chains in the intestine”,Dev. Biol.Jun. 1, 1999 210(1):135-150.
Perreault et al., “Epithelial vs mesenchymal contribution to the extracellular matrix in the human intestine”,Biochem. Biophys. Res. Commun.Jul. 9, 1998 248(1):121-126.
Sun et al., &#

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