Organic acid producing microorganism and process for...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound

Reexamination Certificate

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C435S139000, C435S140000, C435S145000, C435S252100

Reexamination Certificate

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07063968

ABSTRACT:
A novel microorganism capable of producing organic acids,Mannheimiasp. 55E, and a process for producing organic acid through anaerobic and aerobic incubation using the novel microorganism are provided. The method of producing an organic acid using the microorganism involves incubatingMannheimiasp. 55E with Accession Number KCTC 0769BP in a medium under anaerobic or aerobic conditions and obtaining an organic acid from the medium.Mannheimiasp. 55E produces succinic acid, lactic acid, and formic acid under anaerobic conditions saturated with CO2, and succinic acid, lactic acid, acetic acid, and formic acid under aerobic or N2anaerobic conditions.Mannheimiasp. 55E is a facultative anaerobe tolerant of oxygen. Thus, the use ofMannheimiasp. 55E in producing organic acids can eliminate a problem of process instability, which would occur by the presence of oxygen in a fermentation process of producing organic acids using an obligate anaerobic microorganism.

REFERENCES:
“Succinic acid production byAnaerabiospirillum succiniciproducens: effects of the H2/CO2 supply and glucose concentration”; Authors: Pyung Cheon Lee, Woo Gi Lee, Sunhoon Kwon, Sang Yup Lee and Ho Nam Chang; Elsevier Science Inc.; Enzyme and Microbial Technology, vol. 24; 1999; pp. 549-554.
“Anaerobiospirillum, a New Genus of Spiral-Shaped Bacteria”; Authors: C. P. Davis, d. Cleven, J. Brown and E. Balish; International Journal of Systematic Bacteriology; International Association of Microbiological Societies, vol. 26, No. 4; Oct. 1976; pp. 498-504.
“Chemical and Fuel Production by Anaerobic Bacteria”; Author: J. G. Zeikus; Ann. Rev. Microbiol., vol. 34; 1980; pp. 423-464.
International Search Report; International application No. PCT/KR01/01127; International filing date: Jun. 29, 2001; Date of Mailing: Oct. 31, 2001.
International Preliminary Examination Report; International application No. PCT/KR01/01127; International filing date: Jun. 29, 2001; Date of Completion: Oct. 11, 2002.

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