Chemistry: electrical and wave energy – Processes and products – Electrostatic field or electrical discharge
Patent
1992-03-09
1993-05-11
Niebling, John
Chemistry: electrical and wave energy
Processes and products
Electrostatic field or electrical discharge
2041801, 204299R, G01N 2726, G01N 27447
Patent
active
052098346
ABSTRACT:
A method and apparatus are provided for application of electrical field gradients to induce particle velocities to enable particle sequence and identification information to be obtained. Particle sequence is maintained by providing electroosmotic flow for an electrolytic solution in a particle transport tube. The transport tube and electrolytic solution are selected to provide an electroosmotic radius of >100 so that a plug flow profile is obtained for the electrolytic solution in the transport tube. Thus, particles are maintained in the same order in which they are introduced in the transport tube. When the particles also have known electrophoretic velocities, the field gradients introduce an electrophoretic velocity component onto the electroosmotic velocity. The time that the particles pass selected locations along the transport tube may then be detected and the electrophoretic velocity component calculated for particle identification. One particular application is the ordered transport and identification of labeled nucleotides sequentially cleaved from a strand of DNA.
REFERENCES:
patent: 4793705 (1988-12-01), Shera
patent: 4962037 (1990-10-01), Shera et al.
James W. Jorgenson and Krynn DeArman Lukacs, "Capillary Zone Electrophoresis", Science, vol. 222, pp. 266-272.
James W. Jorgenson and Krynn DeAmar Lukacs, "Zone Electrophoresis in Open-Tubular Glass Capillaries" Analytical Chemistry, 53, pp. 1298-1302.
C. L. Rice and R. Whitehead, "Electrokinetic Flow in a Narrow Cylindrical Capillary," Journal of Physical Chemistry 69, pp. 4017-4023.
E. Brooks Shera et al., "Detection of Single Fluorescent Molecules", Chemical Physics Letters, vol. 1-4, No. 6, pp. 553-557.
Gaetjens Paul D.
Moser William R.
Niebling John
Starsiak Jr. John S.
The United States of America as represented by the United States
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